Expression of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP in serosal tissue of intraperitoneal organs and adhesions

Nasser Chegini, Kristina Kotseos, Yong Zhao, Chunfeng Ma, Frederick McLean, Michael Peter Diamond, Lena Holmdahl, James Burns

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Objective: To compare expression of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP (TIMP-1) in serosal tissue of intraperitoneal organs and adhesions. Design: Prospective and cross-sectional study. Setting: Academic research centers. Patient(s): Patients undergoing abdominal or pelvic surgery. Intervention(s): MMP-1 and TIMP-1 expression. Main Outcome Measure(s): Expression of messenger ribonucleic acid (mRNA) and protein was measured by using quantitative reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay. Result(s): Serosal tissue of intraperitoneal organs and adhesions express MMP-1 and TIMP-1 mRNA and protein at levels that are consistently varied with 10- to 10,000-fold and 2- to 10-fold higher TIMP, mRNA and protein, respectively. Parietal peritoneum, fallopian tubes and ovaries express higher MMP-1 mRNA levels compared with uterus and adhesions; the lowest expression is found in small and large bowels, subcutaneous tissue. and omentum. Expression of TIMP-1 mRNA was less variable; the highest level was found in the uterus and the lowest in subcutaneous tissue and small bowels. There was less variability in MMP-1 and TIMP-1 protein content than mRNA expression; ovaries and adhesions contained the highest MMP-1 and TIMP-1 levels, respectively, and peritoneum contained the lowest. The MMP-1 and TIMP-1 content and ratios further indicate limited MMP-1 proteolytic activity. Although tissues from premenopausal women express more MMP-1 and TIMP-1, expression did not differ by sex or age. Conclusion(s): Because MMP-1 and TIMP-1 expression varies consistently among the serosal tissues of peritoneal organs and adhesions, and because tissue injury alters their expression, site-specific variations in expression of these substances may predispose a particular organ to develop more adhesions.

Original languageEnglish (US)
Pages (from-to)1212-1219
Number of pages8
JournalFertility and sterility
Volume76
Issue number6
DOIs
StatePublished - Dec 17 2001
Externally publishedYes

Keywords

  • MMP-1
  • MRNA
  • Peritoneal adhesion
  • Protein
  • Serosal tissue
  • TIMP-1

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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