Expression of peroxisome proliterator-activated receptor (PPARγ) mRNA in adipose and muscle tissue of Holstein and Charolais cattle

P. W. Huff, M. Q. Ren, F. J. Lozeman, R. J. Weselake, J. Wegner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Peroxisome proliferator-activated receptor γ (PPARγ) regulates adipogenesis and lipid metabolism-related gene transcripts. The role, however, of PPARγ in different adipose depots and muscle in Holstein and Charolais cattle is still unclear. We used 20 animals (10 from each breed) for semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) to measure PPARy mRNA levels in subcutaneous (SC), perirenal (PR), omental (OM), and intramuscular (IM) adipose depots as well as longissimus muscle (MU). IM fat was dissected from muscle tissue in MU. Holstein were characterized by their higher OM (P < 0.01) and PR (P < 0.05) fat weights while the Charolais had a higher body weight (P < 0.001) and a larger longissimus muscle area (P < 0.001). The IM fat content and marbling scores tended to be higher in Holstein. No significant differences in PPARy mRNA expression were observed between these two breeds for any tissue. In both breeds, MU PPARγ had the lowest expressed mRNA level (P < 0.05). In the IM fat depot, expression was higher (P < 0.05) than MU, but lower than the SC, PR, and OM fat depot PPARγ mRNA levels. Only OM PPARγ mRNA levels were higher (P < 0.05) than SC and PR in Charolais. To characterise the role of PPARγ mRNA in bovine adipogenesis, correlations were performed among PPARγ mRNA, carcass characteristics, and adipogenesis-related genes.

Original languageEnglish (US)
Pages (from-to)49-55
Number of pages7
JournalCanadian Journal of Animal Science
Volume84
Issue number1
DOIs
StatePublished - Mar 2004

Fingerprint

Peroxisomes
Charolais
peroxisomes
muscle tissues
adipose tissue
Adipose Tissue
Holstein
Muscles
Messenger RNA
receptors
cattle
intramuscular fat
Adipogenesis
Fats
muscles
breeds
longissimus muscle
marbling
Peroxisome Proliferator-Activated Receptors
lipids

Keywords

  • Adipose tissue
  • Cattle
  • Muscle tissue
  • PPARγ

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology

Cite this

Expression of peroxisome proliterator-activated receptor (PPARγ) mRNA in adipose and muscle tissue of Holstein and Charolais cattle. / Huff, P. W.; Ren, M. Q.; Lozeman, F. J.; Weselake, R. J.; Wegner, J.

In: Canadian Journal of Animal Science, Vol. 84, No. 1, 03.2004, p. 49-55.

Research output: Contribution to journalArticle

Huff, P. W. ; Ren, M. Q. ; Lozeman, F. J. ; Weselake, R. J. ; Wegner, J. / Expression of peroxisome proliterator-activated receptor (PPARγ) mRNA in adipose and muscle tissue of Holstein and Charolais cattle. In: Canadian Journal of Animal Science. 2004 ; Vol. 84, No. 1. pp. 49-55.
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