Expression of slc5a8 in kidney and its role in Na+-coupled transport of lactate

Elangovan Gopal, You Jun Fei, Mitsuru Sugawara, Seiji Miyauchi, Lina Zhuang, Pamela Moore Martin, Sylvia B Smith, Puttur D Prasad, Vadivel Ganapathy

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

We report here on the expression of slc5a8 in kidney and its relevance to Na+-coupled reabsorption of lactate. slc5a8 is the murine ortholog of SLC5A8, a candidate tumor suppressor gene, which we recently cloned from human intestine and demonstrated its functional identity as a Na+-coupled transporter for short-chain fatty acids and lactate. The slc5a8 cDNA, cloned from mouse kidney, codes for a protein consisting of 611 amino acids. When expressed heterologously in mammalian cells or Xenopus oocytes, slc5a8 mediates Na+-coupled electrogenic transport of lactate/pyruvate as well as short-chain fatty acids (e.g. acetate, propionate, and butyrate). The Na +/fatty acid stoichiometry varies depending on the fatty acid substrate (2:1 for lactate and 4:1 for propionate). This phenomenon of variable Na+/ substrate stoichiometry depending on the fatty acid substrate is also demonstrable with human SLC5A8. In situ hybridization with sagittal sections of mouse kidney demonstrates abundant expression of the transcripts in the cortex as well as the medulla. Brush border membrane vesicles prepared from rabbit kidney are able to transport lactate in a Na+-coupled manner. The transport process exhibits the overshoot phenomenon, indicating uphill lactate transport in response to the transmembrane Na+ gradient. The Na+-coupled lactate transport in these membrane vesicles is inhibitable by short-chain fatty acids. We conclude that slc5a8 is expressed abundantly in the kidney and that it plays a role in the active reabsorption of lactate. slc5a8 is the first transporter known to be expressed in mammalian kidney that has the ability to mediate the Na+-coupled reabsorption of lactate.

Original languageEnglish (US)
Pages (from-to)44522-44532
Number of pages11
JournalJournal of Biological Chemistry
Volume279
Issue number43
DOIs
StatePublished - Oct 22 2004

Fingerprint

Lactic Acid
Kidney
Volatile Fatty Acids
Fatty Acids
Propionates
Stoichiometry
Substrates
Membranes
Butyrates
Active Biological Transport
Brushes
Microvilli
Xenopus
Tumor Suppressor Genes
Pyruvic Acid
Oocytes
Intestines
In Situ Hybridization
Tumors
Acetates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gopal, E., Fei, Y. J., Sugawara, M., Miyauchi, S., Zhuang, L., Martin, P. M., ... Ganapathy, V. (2004). Expression of slc5a8 in kidney and its role in Na+-coupled transport of lactate. Journal of Biological Chemistry, 279(43), 44522-44532. https://doi.org/10.1074/jbc.M405365200

Expression of slc5a8 in kidney and its role in Na+-coupled transport of lactate. / Gopal, Elangovan; Fei, You Jun; Sugawara, Mitsuru; Miyauchi, Seiji; Zhuang, Lina; Martin, Pamela Moore; Smith, Sylvia B; Prasad, Puttur D; Ganapathy, Vadivel.

In: Journal of Biological Chemistry, Vol. 279, No. 43, 22.10.2004, p. 44522-44532.

Research output: Contribution to journalArticle

Gopal, Elangovan ; Fei, You Jun ; Sugawara, Mitsuru ; Miyauchi, Seiji ; Zhuang, Lina ; Martin, Pamela Moore ; Smith, Sylvia B ; Prasad, Puttur D ; Ganapathy, Vadivel. / Expression of slc5a8 in kidney and its role in Na+-coupled transport of lactate. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 43. pp. 44522-44532.
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