Expression of the mouse WNK1 gene in correlation with ganglioside GD3 and functional analysis of the mouse WNK1 promoter

Guichao Zeng, Luoyi Gao, Tian Xia, Yihua Gu, Robert K Yu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

WNK1 is one of WNK (With No K=Lysine) protein kinases which comprise a newly described subfamily. Our studies showed that expression of the mouse WNK1 gene was dramatically suppressed in a tumor cell line when its phenotype was altered by suppression of the GD3-synthase gene expression. The mouse WNK1 gene was expressed at a high level at early stage of embryonic brain and its expression decreased as brain developed, similar to the expression pattern of the GD3-synthase gene. To study transcriptional regulation, we cloned a 5′-flanking 1239-bp fragment of the mouse WNK1 gene. This fragment contains a number of potential consensus binding sites for transcription factors, including Sp1, AP2, CCAAT, Est-1, Oct-1, CNBP, and NFkB, but lacks a TATA box. Primer extension identified multiple putative transcriptional initiation sites, including several sites downstream of the ATG codon. Activities of the promoter fragments were assessed in mouse breast Sa/R-MT cells by transient transfection and the results showed that the promoter elements between -700 and -977 is required for maintaining a high level of promoter activity of the TATA-less mouse WNK1 gene.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalGene
Volume344
DOIs
StatePublished - Jan 3 2005

Fingerprint

Genes
Sp1 Transcription Factor
TATA Box
Brain
Tumor Cell Line
Codon
Protein Kinases
Lysine
Transfection
Breast
Binding Sites
GD3 ganglioside
Phenotype
Gene Expression

Keywords

  • Ganglioside
  • Gene expression
  • Promoter
  • Protein kinase
  • Transcriptional regulation

ASJC Scopus subject areas

  • Genetics

Cite this

Expression of the mouse WNK1 gene in correlation with ganglioside GD3 and functional analysis of the mouse WNK1 promoter. / Zeng, Guichao; Gao, Luoyi; Xia, Tian; Gu, Yihua; Yu, Robert K.

In: Gene, Vol. 344, 03.01.2005, p. 233-239.

Research output: Contribution to journalArticle

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