Expression of the mutant green fluorescent protein gene in insect cells and larvaae

Zi Fei Pei, Quansheng Du, Yi Peng Qi, Ying Zhu

Research output: Contribution to journalArticle

Abstract

A expression cassette containing the mutant green fluorescent protein gene (gfpS65T) and the intact polyhedrin gene was transposed to the attachment site for Tn7 in AcNPV-Bacmid by using the recombinant transposon vector pFGP, thus resulting in a recombinant baculovirus which carries the gfp gene and another copy of polyhedrin gene. The Sf9 cells infected by the recombinant baculovirus can stably produce polyhedra and show strong green fluorescence under ultraviolet light since the gfpS65T gene expression. Concomtantly, Trichoplusia ni larvae inoculated with the recombinant baculovirus show also green under sunlight and appear stronger green fluorescence under irradiation of a hand-hold long-wavelength ultraviolet light. It is possible that the recombinant baculovirus bearing the reporter gene gfp are able to be useful for studying entomological ecology and baculovirus epidemiology et al.

Original languageEnglish (US)
Pages (from-to)231-232
Number of pages2
JournalWuhan Daxue Xuebao/Journal of Wuhan University
Volume45
Issue number2
StatePublished - Dec 1 1999

Keywords

  • Bac-to-Bac system
  • Baculovirus
  • Gene expression
  • Green fluorescent protein gene

ASJC Scopus subject areas

  • Management of Technology and Innovation

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