Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells

Implications for cellular magnetic resonance imaging

Edyta Pawelczyk, Ali Syed Arbab, Sunil Pandit, Elbert Hu, Joseph A. Frank

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Ferumoxides-protamine sulfate (FE-Pro) complexes are used for intracellular magnetic labeling of cells to non-invasively monitor cell trafficking by in vivo MRI. FE-Pro labeling is non-toxic to cells; however, the effects of FE-Pro labeling on cellular expression of transferrin receptor (TfR- 1) and ferritin, proteins involved in iron transport and storage, has not been reported. FE-Pro-labeled human mesenchymal stem cells (MSCs), HeLa cells and primary macrophages were cultured from 1 week to 2 months and evaluated for TfR- 1 and ferritin gene expression by RT-PCR and protein levels were determined using Western blots. MTT (proliferation assay) and reactive oxygen species (ROS) analysis were performed. FE-Pro labeling of HeLa and MSCs resulted in a transient decrease in TfR- 1 mRNA and protein levels. In contrast, Fe-Pro labeling of primary macrophages resulted in an increase in TfR-1 mRNA but not in TfR-1 protein levels. Ferritin mRNA and protein levels increased transiently in labeled HeLa and macrophages but were sustained in MSCs. No changes in MTT and ROS analysis were noted. In conclusion, FE-Pro labeling elicited physiological changes of iron metabolism or storage, validating the safety of this procedure for cellular tracking by MRI.

Original languageEnglish (US)
Pages (from-to)581-592
Number of pages12
JournalNMR in Biomedicine
Volume19
Issue number5
DOIs
StatePublished - Aug 1 2006
Externally publishedYes

Fingerprint

Protamines
Transferrin Receptors
Magnetic resonance
Ferritins
Labeling
Magnetic Resonance Imaging
Imaging techniques
Macrophages
Stem cells
Mesenchymal Stromal Cells
Proteins
Magnetic resonance imaging
Messenger RNA
Reactive Oxygen Species
Iron
HeLa Cells
Metabolism
Gene expression
ferumoxides
Assays

Keywords

  • Cell labeling
  • Ferritin
  • Ferumoxides
  • Mesenchymal stem cells
  • Primary macrophages
  • Protamine sulfate
  • SPIO
  • Transferrin receptor (TfR-1)

ASJC Scopus subject areas

  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells : Implications for cellular magnetic resonance imaging. / Pawelczyk, Edyta; Arbab, Ali Syed; Pandit, Sunil; Hu, Elbert; Frank, Joseph A.

In: NMR in Biomedicine, Vol. 19, No. 5, 01.08.2006, p. 581-592.

Research output: Contribution to journalArticle

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