Expression pattern of sigma receptor 1 mRNA and protein in mammalian retina

M. Shamsul Ola, Pamela Moore Martin, Amira El-Sherbeny, Penny Roon, Neeraj Agarwal, Vijay P. Sarthy, Pierre Casellas, Vadivel Ganapathy, Sylvia B Smith

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Sigma receptors are nonopiate and nonphencyclidine binding sites that are thought to be neuroprotective due to modulation of N-methyl-D-aspartate (NMDA) receptors. Sigma receptor 1 expression has been demonstrated in numerous tissues including brain. Recently, studies using binding assays have demonstrated sigma receptor 1 in neural retina, however these studies did not demonstrate in which retinal cell type(s) sigma receptor 1 was present nor did they establish unequivocally the molecular identity of the receptor. The present study was designed to address these issues. Reverse transcription-polymerase chain reaction (RT-PCR) analysis amplified sigma receptor 1 in neural retina, RPE-choroid complex, and lens isolated from mice. A similar RT-PCR product was amplified also in three cultured cell lines, rat Müller cells, rat ganglion cells and human ARPE-19 cells. In situ hybridization analysis revealed abundant sigma receptor 1 expression in ganglion cells, cells of the inner nuclear layer, inner segments of photoreceptor cells and retinal pigment epithelial (RPE) cells. Immunohistochemical studies detected the sigma receptor 1 protein in retinal ganglion, photoreceptor, RPE cells and surrounding the soma of cells in the inner nuclear layer. These data provide the first cellular localization of sigma receptor 1 in neural retina and establish the molecular identity of sigma receptor 1 in retinal cells. The demonstration that sigma receptor 1 is present in ganglion cells is particularly noteworthy given the well-documented susceptibility of these cells to glutamate toxicity. Our findings suggest that retinal ganglion cells may be amenable to the neuroprotective effects of sigma ligands under conditions of neurotoxicity such as occurs in diabetes.

Original languageEnglish (US)
Pages (from-to)86-95
Number of pages10
JournalMolecular Brain Research
Volume95
Issue number1-2
DOIs
StatePublished - Nov 1 2001

Fingerprint

Retina
Messenger RNA
Proteins
Ganglia
Retinal Pigments
Reverse Transcription
Epithelial Cells
sigma-1 receptor
sigma Receptors
Polymerase Chain Reaction
Photoreceptor Cells
Vertebrate Photoreceptor Cells
Choroid
Retinal Ganglion Cells
Carisoprodol
Neuroprotective Agents
N-Methyl-D-Aspartate Receptors
Lenses
In Situ Hybridization
Glutamic Acid

Keywords

  • Ciliary body
  • Ganglion cell
  • Retina
  • Retinal pigment epithelial cell
  • Sigma receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Expression pattern of sigma receptor 1 mRNA and protein in mammalian retina. / Shamsul Ola, M.; Martin, Pamela Moore; El-Sherbeny, Amira; Roon, Penny; Agarwal, Neeraj; Sarthy, Vijay P.; Casellas, Pierre; Ganapathy, Vadivel; Smith, Sylvia B.

In: Molecular Brain Research, Vol. 95, No. 1-2, 01.11.2001, p. 86-95.

Research output: Contribution to journalArticle

Shamsul Ola, M, Martin, PM, El-Sherbeny, A, Roon, P, Agarwal, N, Sarthy, VP, Casellas, P, Ganapathy, V & Smith, SB 2001, 'Expression pattern of sigma receptor 1 mRNA and protein in mammalian retina', Molecular Brain Research, vol. 95, no. 1-2, pp. 86-95. https://doi.org/10.1016/S0169-328X(01)00249-2
Shamsul Ola, M. ; Martin, Pamela Moore ; El-Sherbeny, Amira ; Roon, Penny ; Agarwal, Neeraj ; Sarthy, Vijay P. ; Casellas, Pierre ; Ganapathy, Vadivel ; Smith, Sylvia B. / Expression pattern of sigma receptor 1 mRNA and protein in mammalian retina. In: Molecular Brain Research. 2001 ; Vol. 95, No. 1-2. pp. 86-95.
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