TY - JOUR
T1 - Extensive association analysis between polymorphisms of PON gene cluster with coronary heart disease in Chinese Han population
AU - Wang, Xiaoling
AU - Fan, Zhongjie
AU - Huang, Jianfeng
AU - Su, Shaoyong
AU - Yu, Quanjun
AU - Zhao, Jiangong
AU - Hui, Rutai
AU - Yao, Zhijian
AU - Shen, Yan
AU - Qiang, Boqin
AU - Gu, Dongfeng
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Objective - An extensive association analysis of PON gene cluster (PONs) with coronary heart disease (CHD) was performed in Chinese Han population. Methods and Results - Thirty polymorphisms of PON1, PON2, and PON3 gene were identified by direct sequencing of genomic DNA derived from 48 randomly selected patients. Twelve polymorphisms were additionally investigated for association with CHD in 474 male patients and 475 controls. Univariate analyses showed the cases had significantly higher frequencies of PON1 192Q allele, 160R allele, - 162A allele, and PON2 311C allele than were seen in the controls. Logistic regression analyses revealed only the PON1 R160G and -162G/A polymorphisms remained significantly associated with CHD (P=0.0054 and P=0.0002). Haplotype analyses for various polymorphism combinations additionally confirmed the results of individual polymorphism analyses. Only the frequencies of haplotypes containing - 162A allele were significantly higher, whereas only the frequencies of haplotypes containing 160G allele were significantly lower in cases than in controls in various polymorphism combinations. Conclusions - This extensive association study has identified the PON1 - 162G/A and R160G polymorphisms to be independently associated with CHD in Chinese Han population and warrants additional study to elucidate the biological mechanism.
AB - Objective - An extensive association analysis of PON gene cluster (PONs) with coronary heart disease (CHD) was performed in Chinese Han population. Methods and Results - Thirty polymorphisms of PON1, PON2, and PON3 gene were identified by direct sequencing of genomic DNA derived from 48 randomly selected patients. Twelve polymorphisms were additionally investigated for association with CHD in 474 male patients and 475 controls. Univariate analyses showed the cases had significantly higher frequencies of PON1 192Q allele, 160R allele, - 162A allele, and PON2 311C allele than were seen in the controls. Logistic regression analyses revealed only the PON1 R160G and -162G/A polymorphisms remained significantly associated with CHD (P=0.0054 and P=0.0002). Haplotype analyses for various polymorphism combinations additionally confirmed the results of individual polymorphism analyses. Only the frequencies of haplotypes containing - 162A allele were significantly higher, whereas only the frequencies of haplotypes containing 160G allele were significantly lower in cases than in controls in various polymorphism combinations. Conclusions - This extensive association study has identified the PON1 - 162G/A and R160G polymorphisms to be independently associated with CHD in Chinese Han population and warrants additional study to elucidate the biological mechanism.
KW - Coronary heart disease
KW - Genetics
KW - LDL oxidation
KW - Linkage disequilibrium
KW - Paraoxonase
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U2 - 10.1161/01.ATV.0000051702.38086.C1
DO - 10.1161/01.ATV.0000051702.38086.C1
M3 - Article
C2 - 12588779
AN - SCOPUS:0037324353
SN - 1079-5642
VL - 23
SP - 328
EP - 334
JO - Arteriosclerosis, thrombosis, and vascular biology
JF - Arteriosclerosis, thrombosis, and vascular biology
IS - 2
ER -