Ezrin and osteonectin, two proteins associated with cell shape and growth, are enriched in the locus coeruleus

Clare M Bergson, Hai Qing Zhao, Kiyofumi Saijoh, Ronald S. Duman, Eric J. Nestler

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In an attempt to characterize proteins which are enriched or specifically expressed in the locus coeruleus (LC), a region of the brain which plays a critical role in opiate dependence and withdrawal, we have screened a bovine LC cDNA library with an LC minus cerebellum subtracted cDNA probe and isolated several (38) positively hybridizing clones. DNA sequence analysis revealed that two of the clones encoded ezrin and osteonectin, proteins normally associated with cell growth and morphology in peripheral tissues. Regional northern blots from bovine brain and in situ hybridization studies in rat show that ezrin is expressed at high levels in the LC with only low levels detectable in other brain regions. Osteonectin is also abundant in the LC, but in contrast to ezrin, is expressed at high levels in the dorsal raphe and substantia niera. The results indicate that two proteins, ezrin and osteonectin, are highly enriched in the LC. This raises the possibility that these polypeptides, which play a role in the morphological response of some non-neuronal cell types to growth factors and to other intracellular signals, may also regulate these properties in noradrenergic and other selected neurons in the brain.

Original languageEnglish (US)
Pages (from-to)64-73
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume4
Issue number1
DOIs
StatePublished - Apr 1 1993
Externally publishedYes

Fingerprint

Osteonectin
Locus Coeruleus
Cell Shape
Growth
Proteins
Brain
Clone Cells
Opioid-Related Disorders
Gene Library
DNA Sequence Analysis
Northern Blotting
Cerebellum
In Situ Hybridization
ezrin
Intercellular Signaling Peptides and Proteins
Complementary DNA
Neurons
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Ezrin and osteonectin, two proteins associated with cell shape and growth, are enriched in the locus coeruleus. / Bergson, Clare M; Zhao, Hai Qing; Saijoh, Kiyofumi; Duman, Ronald S.; Nestler, Eric J.

In: Molecular and Cellular Neuroscience, Vol. 4, No. 1, 01.04.1993, p. 64-73.

Research output: Contribution to journalArticle

Bergson, Clare M ; Zhao, Hai Qing ; Saijoh, Kiyofumi ; Duman, Ronald S. ; Nestler, Eric J. / Ezrin and osteonectin, two proteins associated with cell shape and growth, are enriched in the locus coeruleus. In: Molecular and Cellular Neuroscience. 1993 ; Vol. 4, No. 1. pp. 64-73.
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