FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1

Jianqiu Zou, Fen Tian, Ji Li, Wyatt Pickner, Molly Long, Khosrow Rezvani, Hongmin Wang, Dong Zhang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

DNA damage response (DDR) and the centrosome cycle are two of the most critical processes for maintaining a stable genome in animals. Sporadic evidence suggests a connection between these two processes. Here, we report our findings that six Fanconi Anemia (FA) proteins, including FancI and FancJ, localize to the centrosome. Intriguingly, we found that the localization of FancJ to the mother centrosome is stimulated by a DNA interstrand crosslinker, Mitomycin C (MMC). We further show that, in addition to its role in interstrand crosslinking (ICL) repair, FancJ also regulates the normal centrosome cycle as well as ICL induced centrosome amplification by activating the polo-like kinase 1 (PLK1). We have uncovered a novel function of FancJ in centrosome biogenesis and established centrosome amplification as an integral part of the ICL response.

Original languageEnglish (US)
Pages (from-to)1022-1031
Number of pages10
JournalBiology Open
Volume2
Issue number10
DOIs
StatePublished - Oct 15 2013
Externally publishedYes

Fingerprint

centrosomes
Centrosome
Crosslinking
Amplification
phosphotransferases (kinases)
Chemical activation
Fanconi Anemia Complementation Group Proteins
crosslinking
DNA
Mitomycin
Animals
Repair
Genes
mitomycin
polo-like kinase 1
DNA damage
DNA Damage
Genome
genome

Keywords

  • Centrosome
  • FancJ
  • PLK1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1. / Zou, Jianqiu; Tian, Fen; Li, Ji; Pickner, Wyatt; Long, Molly; Rezvani, Khosrow; Wang, Hongmin; Zhang, Dong.

In: Biology Open, Vol. 2, No. 10, 15.10.2013, p. 1022-1031.

Research output: Contribution to journalArticle

Zou, Jianqiu ; Tian, Fen ; Li, Ji ; Pickner, Wyatt ; Long, Molly ; Rezvani, Khosrow ; Wang, Hongmin ; Zhang, Dong. / FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1. In: Biology Open. 2013 ; Vol. 2, No. 10. pp. 1022-1031.
@article{777550f821fe45fda6679981aafc5f98,
title = "FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1",
abstract = "DNA damage response (DDR) and the centrosome cycle are two of the most critical processes for maintaining a stable genome in animals. Sporadic evidence suggests a connection between these two processes. Here, we report our findings that six Fanconi Anemia (FA) proteins, including FancI and FancJ, localize to the centrosome. Intriguingly, we found that the localization of FancJ to the mother centrosome is stimulated by a DNA interstrand crosslinker, Mitomycin C (MMC). We further show that, in addition to its role in interstrand crosslinking (ICL) repair, FancJ also regulates the normal centrosome cycle as well as ICL induced centrosome amplification by activating the polo-like kinase 1 (PLK1). We have uncovered a novel function of FancJ in centrosome biogenesis and established centrosome amplification as an integral part of the ICL response.",
keywords = "Centrosome, FancJ, PLK1",
author = "Jianqiu Zou and Fen Tian and Ji Li and Wyatt Pickner and Molly Long and Khosrow Rezvani and Hongmin Wang and Dong Zhang",
year = "2013",
month = "10",
day = "15",
doi = "10.1242/bio.20135801",
language = "English (US)",
volume = "2",
pages = "1022--1031",
journal = "Biology Open",
issn = "2046-6390",
publisher = "Company of Biologists Ltd",
number = "10",

}

TY - JOUR

T1 - FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1

AU - Zou, Jianqiu

AU - Tian, Fen

AU - Li, Ji

AU - Pickner, Wyatt

AU - Long, Molly

AU - Rezvani, Khosrow

AU - Wang, Hongmin

AU - Zhang, Dong

PY - 2013/10/15

Y1 - 2013/10/15

N2 - DNA damage response (DDR) and the centrosome cycle are two of the most critical processes for maintaining a stable genome in animals. Sporadic evidence suggests a connection between these two processes. Here, we report our findings that six Fanconi Anemia (FA) proteins, including FancI and FancJ, localize to the centrosome. Intriguingly, we found that the localization of FancJ to the mother centrosome is stimulated by a DNA interstrand crosslinker, Mitomycin C (MMC). We further show that, in addition to its role in interstrand crosslinking (ICL) repair, FancJ also regulates the normal centrosome cycle as well as ICL induced centrosome amplification by activating the polo-like kinase 1 (PLK1). We have uncovered a novel function of FancJ in centrosome biogenesis and established centrosome amplification as an integral part of the ICL response.

AB - DNA damage response (DDR) and the centrosome cycle are two of the most critical processes for maintaining a stable genome in animals. Sporadic evidence suggests a connection between these two processes. Here, we report our findings that six Fanconi Anemia (FA) proteins, including FancI and FancJ, localize to the centrosome. Intriguingly, we found that the localization of FancJ to the mother centrosome is stimulated by a DNA interstrand crosslinker, Mitomycin C (MMC). We further show that, in addition to its role in interstrand crosslinking (ICL) repair, FancJ also regulates the normal centrosome cycle as well as ICL induced centrosome amplification by activating the polo-like kinase 1 (PLK1). We have uncovered a novel function of FancJ in centrosome biogenesis and established centrosome amplification as an integral part of the ICL response.

KW - Centrosome

KW - FancJ

KW - PLK1

UR - http://www.scopus.com/inward/record.url?scp=84979643803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979643803&partnerID=8YFLogxK

U2 - 10.1242/bio.20135801

DO - 10.1242/bio.20135801

M3 - Article

AN - SCOPUS:84979643803

VL - 2

SP - 1022

EP - 1031

JO - Biology Open

JF - Biology Open

SN - 2046-6390

IS - 10

ER -