FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1

Jianqiu Zou, Fen Tian, Ji Li, Wyatt Pickner, Molly Long, Khosrow Rezvani, Hongmin Wang, Dong Zhang

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

DNA damage response (DDR) and the centrosome cycle are two of the most critical processes for maintaining a stable genome in animals. Sporadic evidence suggests a connection between these two processes. Here, we report our findings that six Fanconi Anemia (FA) proteins, including FancI and FancJ, localize to the centrosome. Intriguingly, we found that the localization of FancJ to the mother centrosome is stimulated by a DNA interstrand crosslinker, Mitomycin C (MMC). We further show that, in addition to its role in interstrand crosslinking (ICL) repair, FancJ also regulates the normal centrosome cycle as well as ICL induced centrosome amplification by activating the polo-like kinase 1 (PLK1). We have uncovered a novel function of FancJ in centrosome biogenesis and established centrosome amplification as an integral part of the ICL response.

Original languageEnglish (US)
Pages (from-to)1022-1031
Number of pages10
JournalBiology Open
Volume2
Issue number10
DOIs
StatePublished - Oct 15 2013
Externally publishedYes

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Keywords

  • Centrosome
  • FancJ
  • PLK1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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