Fatty Acids Differentially Modulate Insulin-Stimulated Endothelial Nitric Oxide Production by an Akt-Independent Pathway

Matthew A Lynn, Heidi L. Rupnow, Dean J. Kleinhenz, William A. Kanner, Samuel C. Dudley, C. Michael Hart

Research output: Contribution to journalArticle

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Abstract

Background: Insulin increases endothelial nitric oxide (NO) production by activating endothelial nitric oxide synthase (eNOS) through protein kinase B (Akt)-mediated phosphorylation of serine residue 1179 (p-eNOS serine 1179). Because fatty acids modulate insulin-stimulated Akt signaling cascades in smooth muscle cells, we hypothesized that fatty acids would differentially regulate endothelial Akt signaling, eNOS phosphorylation, and NO production. Methods: Porcine pulmonary artery endothelial cells (PAECs) were treated for 3 hours with 100 μM oleic (18:1) or eicosapentaenoic (20:5) acids or with an equivalent volume of ethanol vehicle (0.1%). PAECs were then treated with graded concentrations (10-9-10-5 M) of insulin or incubated overnight (24 hours) in culture medium without fatty acids before insulin treatment. Activation and phosphorylation of Akt and eNOS were determined by immunoblotting. NO production was measured with a chemiluminescence NO analyzer or with a NO-selective carbon fiber microelectrode. Results: Insulin-stimulated Akt phosphorylation, eNOS phosphorylation, and NO production. The phosphatidylinositol-3 kinase inhibitor wortmannin attenuated insulin-stimulated Akt activation and NO production. Treatment with the ω-3 fatty acid 20:5, but not 18:1, enhanced insulin-stimulated NO production but failed to alter insulin-stimulated Akt activation or eNOS serine 1179 phosphorylation. Conclusion: Individual fatty acyl species have distinct effects on insulin-stimulated endothelial NO production. Although fatty acids alter Akt signaling in muscle cells, the current results indicate that fatty acids do not modulate endothelial NO production through alterations in insulin-stimulated, Akt-mediated eNOS phosphorylation.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalJournal of Investigative Medicine
Volume52
Issue number2
StatePublished - Jan 1 2004

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Phosphorylation
Nitric Oxide
Nitric Oxide Synthase Type III
Fatty Acids
Insulin
Serine
Chemical activation
Endothelial cells
Pulmonary Artery
Muscle
Endothelial Cells
Cells
Phosphatidylinositol 3-Kinase
Proto-Oncogene Proteins c-akt
Chemiluminescence
Microelectrodes
Luminescence
Immunoblotting
Muscle Cells
Smooth Muscle Myocytes

Keywords

  • Endothelial cell
  • Fatty acid
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lynn, M. A., Rupnow, H. L., Kleinhenz, D. J., Kanner, W. A., Dudley, S. C., & Hart, C. M. (2004). Fatty Acids Differentially Modulate Insulin-Stimulated Endothelial Nitric Oxide Production by an Akt-Independent Pathway. Journal of Investigative Medicine, 52(2), 129-136.

Fatty Acids Differentially Modulate Insulin-Stimulated Endothelial Nitric Oxide Production by an Akt-Independent Pathway. / Lynn, Matthew A; Rupnow, Heidi L.; Kleinhenz, Dean J.; Kanner, William A.; Dudley, Samuel C.; Hart, C. Michael.

In: Journal of Investigative Medicine, Vol. 52, No. 2, 01.01.2004, p. 129-136.

Research output: Contribution to journalArticle

Lynn, MA, Rupnow, HL, Kleinhenz, DJ, Kanner, WA, Dudley, SC & Hart, CM 2004, 'Fatty Acids Differentially Modulate Insulin-Stimulated Endothelial Nitric Oxide Production by an Akt-Independent Pathway', Journal of Investigative Medicine, vol. 52, no. 2, pp. 129-136.
Lynn, Matthew A ; Rupnow, Heidi L. ; Kleinhenz, Dean J. ; Kanner, William A. ; Dudley, Samuel C. ; Hart, C. Michael. / Fatty Acids Differentially Modulate Insulin-Stimulated Endothelial Nitric Oxide Production by an Akt-Independent Pathway. In: Journal of Investigative Medicine. 2004 ; Vol. 52, No. 2. pp. 129-136.
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