Fatty acyl-CoA dehydrogenase enzymes in human skeletal muscle

James E. Carroll; Byron S. McGuire; Carole L. Hall

doi:10.1016/0009-8981(86)90017-3

Fatty acyl-CoA dehydrogenase enzymes in human skeletal muscle

James E. Carroll, Byron S. McGuire, Carole L. Hall

Child Neurology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

An electrophoretic and enzyme-substrate staining technique for acyl-CoA dehydrogenase (ACD) enzymes was developed for use with small (< 100 mg) tissue samples. Based on their electrophoretic mobility and substrate staining specificity, at least two and perhaps three chain-length specific enzymes for dehydrogenation of saturated fatty acids were found in human skeletal muscle. ACD enzymes staining with octanoyl-CoA or palmitoyl-CoA were identified by this technique in human skeletal muscle, heart, and liver, but the ACD enzyme staining with butyryl-CoA was difficult to detect and was definitely visualized only in skeletal muscle.

Original language	English (US)
Pages (from-to)	327-333
Number of pages	7
Journal	Clinica Chimica Acta
Volume	161
Issue number	3
DOIs	https://doi.org/10.1016/0009-8981(86)90017-3
State	Published - Dec 30 1986

Keywords

Acyl-CoA dehydrogenase
Heart
Liver
Skeletal muscle

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

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10.1016/0009-8981(86)90017-3

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title = "Fatty acyl-CoA dehydrogenase enzymes in human skeletal muscle",

abstract = "An electrophoretic and enzyme-substrate staining technique for acyl-CoA dehydrogenase (ACD) enzymes was developed for use with small (< 100 mg) tissue samples. Based on their electrophoretic mobility and substrate staining specificity, at least two and perhaps three chain-length specific enzymes for dehydrogenation of saturated fatty acids were found in human skeletal muscle. ACD enzymes staining with octanoyl-CoA or palmitoyl-CoA were identified by this technique in human skeletal muscle, heart, and liver, but the ACD enzyme staining with butyryl-CoA was difficult to detect and was definitely visualized only in skeletal muscle.",

keywords = "Acyl-CoA dehydrogenase, Heart, Liver, Skeletal muscle",

author = "Carroll, {James E.} and McGuire, {Byron S.} and Hall, {Carole L.}",

note = "Funding Information: * This work was supported in part by a grant from the Muscular Dystrophy Association. Correspondence to: James E. Carroll, M.D., Professor and Chief, Pediatric Neurology, CK 297. Medical College of Georgia,{\textquoteleft}Augusta, GA, 30912-3760, USA. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1986",

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day = "30",

doi = "10.1016/0009-8981(86)90017-3",

language = "English (US)",

volume = "161",

pages = "327--333",

journal = "Clinica Chimica Acta",

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TY - JOUR

T1 - Fatty acyl-CoA dehydrogenase enzymes in human skeletal muscle

AU - Carroll, James E.

AU - McGuire, Byron S.

AU - Hall, Carole L.

N1 - Funding Information: * This work was supported in part by a grant from the Muscular Dystrophy Association. Correspondence to: James E. Carroll, M.D., Professor and Chief, Pediatric Neurology, CK 297. Medical College of Georgia,‘Augusta, GA, 30912-3760, USA. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1986/12/30

Y1 - 1986/12/30

N2 - An electrophoretic and enzyme-substrate staining technique for acyl-CoA dehydrogenase (ACD) enzymes was developed for use with small (< 100 mg) tissue samples. Based on their electrophoretic mobility and substrate staining specificity, at least two and perhaps three chain-length specific enzymes for dehydrogenation of saturated fatty acids were found in human skeletal muscle. ACD enzymes staining with octanoyl-CoA or palmitoyl-CoA were identified by this technique in human skeletal muscle, heart, and liver, but the ACD enzyme staining with butyryl-CoA was difficult to detect and was definitely visualized only in skeletal muscle.

AB - An electrophoretic and enzyme-substrate staining technique for acyl-CoA dehydrogenase (ACD) enzymes was developed for use with small (< 100 mg) tissue samples. Based on their electrophoretic mobility and substrate staining specificity, at least two and perhaps three chain-length specific enzymes for dehydrogenation of saturated fatty acids were found in human skeletal muscle. ACD enzymes staining with octanoyl-CoA or palmitoyl-CoA were identified by this technique in human skeletal muscle, heart, and liver, but the ACD enzyme staining with butyryl-CoA was difficult to detect and was definitely visualized only in skeletal muscle.

KW - Acyl-CoA dehydrogenase

KW - Heart

KW - Liver

KW - Skeletal muscle

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JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 3

ER -

Fatty acyl-CoA dehydrogenase enzymes in human skeletal muscle

Abstract

Keywords

ASJC Scopus subject areas

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