Fc-receptor-mediated phagocytosis occurs in macrophages without an increase in average [Ca++](i)

Paul L McNeil, J. A. Swanson, S. D. Wright, S. C. Silverstein, D. L. Taylor

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Abstract

The calcium ion has been implicated as a cytosolic signal or regulator in phagocytosis. Using the Ca++-sensitive photoprotein aequorin we have measured intracellular free Ca++ ion concentration ([Ca++](i)) in thioglycolate-elicited mouse peritoneal macrophages during phagocytosis and IgG-induced spreading. Macrophages plated on glass were loaded with aequorin and [Ca++](i) was then measured from cell populations, both as previously described (McNeil, P.L., and D.L. Taylor, 1985, Cell Calcium, 6:83-92). Aequorin indicated a resting [Ca++](i) in adherent macrophages of 84 nM and was responsive to changes in [Ca++](i) induced by the addition of Mg-ATP (0.1 mM) or serum to medium. However, during the 15 min required for phagocytosis of seven or eight IgG-coated erythrocytes per macrophage loaded with aequorin, we measured no change in [Ca++](i). Similarly, the ligation of Fc-receptors that occurs when macrophages spread on immune complex-coated coverslips did not change macrophage [Ca++](i). In contrast, a rise in [Ca++](i) of macrophages was measured during phagocytosis occurring in a serum-free saline of pH 7.85, and as a consequence of incubation with quin2 A/M. We estimate that had a change in [Ca++](i) occurred during phagocytosis, aequorin would have detected a rise from 0.1 to 1.0 μM taking place in as little as 2% of the macrophage's cytoplasmic volume. We therefore suggest that either Ca++ is not involved as a cytoplasmic signal for phagocytosis or that increases in [Ca++](i) during phagocytosis are confined to such small regions of cytoplasm as to be below the limits of detection by our cellular averaging method. Our data emphasizes, moreover, the need for well-defined, nonperturbing conditions in such measurements of [Ca++](i).

Original languageEnglish (US)
Pages (from-to)1586-1592
Number of pages7
JournalJournal of Cell Biology
Volume102
Issue number5
DOIs
StatePublished - Jan 1 1986

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Fc Receptors
Aequorin
Phagocytosis
Macrophages
Immunoglobulin G
Luminescent Proteins
Ions
Calcium
Thioglycolates
Peritoneal Macrophages
Antigen-Antibody Complex
Serum
Glass
Ligation
Limit of Detection
Cytoplasm
Adenosine Triphosphate
Erythrocytes
Population

ASJC Scopus subject areas

  • Cell Biology

Cite this

McNeil, P. L., Swanson, J. A., Wright, S. D., Silverstein, S. C., & Taylor, D. L. (1986). Fc-receptor-mediated phagocytosis occurs in macrophages without an increase in average [Ca++](i). Journal of Cell Biology, 102(5), 1586-1592. https://doi.org/10.1083/jcb.102.5.1586

Fc-receptor-mediated phagocytosis occurs in macrophages without an increase in average [Ca++](i). / McNeil, Paul L; Swanson, J. A.; Wright, S. D.; Silverstein, S. C.; Taylor, D. L.

In: Journal of Cell Biology, Vol. 102, No. 5, 01.01.1986, p. 1586-1592.

Research output: Contribution to journalArticle

McNeil, PL, Swanson, JA, Wright, SD, Silverstein, SC & Taylor, DL 1986, 'Fc-receptor-mediated phagocytosis occurs in macrophages without an increase in average [Ca++](i)', Journal of Cell Biology, vol. 102, no. 5, pp. 1586-1592. https://doi.org/10.1083/jcb.102.5.1586
McNeil, Paul L ; Swanson, J. A. ; Wright, S. D. ; Silverstein, S. C. ; Taylor, D. L. / Fc-receptor-mediated phagocytosis occurs in macrophages without an increase in average [Ca++](i). In: Journal of Cell Biology. 1986 ; Vol. 102, No. 5. pp. 1586-1592.
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