Fc receptors for IgG, IgM and IgE on human leukaemic lymphocytes

H. L. Spiegelberg, Paul M Dainer

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Fc receptors for IgG, IgM, IgE and the cell surface immunoglobulins (SIg) were analysed on lymphocytes from seventeen patients with chronic lymphatic leukaemia (CLL), one with lymphosarcoma cell leukaemia (LSL), two each with hairy cell leukaemia (HCL), acute lymphatic leukaemia (ALL) and Sezary syndrome. Fc receptors for IgG and IgM were detected by rosette formation with ox erythrocytes (E(O)) sensitized with rabbit IgG (E(O)A(G)) and IgM (E(O)A(M)) anti-E(O) antibodies, respectively. Fc receptors for IgE were analysed either with E(O) coated with glutaraldehyde coupled complexes consisting of rabbit Fab' fragments of anti-E(O) antibodies and Fc fragments of an IgE myeloma protein (E(O)A(E)), or with aldehyde fixed E(O) to which IgE was absorbed. SIg of classes IgM, IgD, IgG and κ and λ light chain were detected with E(O) coated with complexes consisting of Fab'-anti-E(O) and purified F(ab')2 fragments of specific goat antibodies. Lymphocytes of all patients with CLL, LSL and HCL had Fc receptors for IgG (65 ± 15% E(O)A(G)+, normal 22.0 ± 5.8%). Ten patients had significant numbers of cells with IgM Fc receptors (37 ± 22% E(O)A(M)+, normal 1.2 ± 1.5%) which were detected without overnight culturing of the lymphocytes. Lymphocytes of four patients (two CLL, one LSL, one HCL) had Fc receptors for IgE (22 - 88% E(O)A(E)+, normal 1.8 ± 0.7%). The cells of three of these four patients were also E(O)A(M)+. The high numbers of rosetting cells indicated that individual lymphocytes must have carried more than one class of Fc receptors. The lymphocytes of the ALL and Sezary syndrome patients had few Fc receptor positive cells. Of the seventeen patients with CLL, twelve were SIgM+ and/or SIgD+, only four κ+ or λ+ and one had no SIg. The cells of the LSL and one of the HCL patients were SIgM+ and SIgD+, whilst the cells of the other HCL patient were SIgG, λ+. None of the other patients had more than 10% SIgG+ cells. The ALL and Sezary syndrome patients had low numbers of SIg+ and Fc receptor positive cells. These data indicate that lymphocytes of patients with B-cell leukaemias can carry different classes of Fc receptors simultaneously; the different classes are found in a decreasing frequency of IgG > IgM > IgE.

Original languageEnglish (US)
Pages (from-to)286-295
Number of pages10
JournalClinical and Experimental Immunology
Volume35
Issue number2
StatePublished - Jan 1 1979
Externally publishedYes

Fingerprint

Immunoglobulin E
Immunoglobulin G
Erythrocytes
Lymphocytes
Fc Receptors
Hairy Cell Leukemia
B-Cell Antigen Receptors
B-Cell Chronic Lymphocytic Leukemia
Sezary Syndrome
Non-Hodgkin's Lymphoma
Leukemia
Immunoglobulin M
immunoglobulin M receptor
Anti-Idiotypic Antibodies
Cell Count
B-Cell Leukemia
Rabbits
Immunoglobulin Fc Fragments
Myeloma Proteins
Rosette Formation

ASJC Scopus subject areas

  • Immunology

Cite this

Fc receptors for IgG, IgM and IgE on human leukaemic lymphocytes. / Spiegelberg, H. L.; Dainer, Paul M.

In: Clinical and Experimental Immunology, Vol. 35, No. 2, 01.01.1979, p. 286-295.

Research output: Contribution to journalArticle

@article{e75d418fdded45d08b527be5bd1ec870,
title = "Fc receptors for IgG, IgM and IgE on human leukaemic lymphocytes",
abstract = "Fc receptors for IgG, IgM, IgE and the cell surface immunoglobulins (SIg) were analysed on lymphocytes from seventeen patients with chronic lymphatic leukaemia (CLL), one with lymphosarcoma cell leukaemia (LSL), two each with hairy cell leukaemia (HCL), acute lymphatic leukaemia (ALL) and Sezary syndrome. Fc receptors for IgG and IgM were detected by rosette formation with ox erythrocytes (E(O)) sensitized with rabbit IgG (E(O)A(G)) and IgM (E(O)A(M)) anti-E(O) antibodies, respectively. Fc receptors for IgE were analysed either with E(O) coated with glutaraldehyde coupled complexes consisting of rabbit Fab' fragments of anti-E(O) antibodies and Fc fragments of an IgE myeloma protein (E(O)A(E)), or with aldehyde fixed E(O) to which IgE was absorbed. SIg of classes IgM, IgD, IgG and κ and λ light chain were detected with E(O) coated with complexes consisting of Fab'-anti-E(O) and purified F(ab')2 fragments of specific goat antibodies. Lymphocytes of all patients with CLL, LSL and HCL had Fc receptors for IgG (65 ± 15{\%} E(O)A(G)+, normal 22.0 ± 5.8{\%}). Ten patients had significant numbers of cells with IgM Fc receptors (37 ± 22{\%} E(O)A(M)+, normal 1.2 ± 1.5{\%}) which were detected without overnight culturing of the lymphocytes. Lymphocytes of four patients (two CLL, one LSL, one HCL) had Fc receptors for IgE (22 - 88{\%} E(O)A(E)+, normal 1.8 ± 0.7{\%}). The cells of three of these four patients were also E(O)A(M)+. The high numbers of rosetting cells indicated that individual lymphocytes must have carried more than one class of Fc receptors. The lymphocytes of the ALL and Sezary syndrome patients had few Fc receptor positive cells. Of the seventeen patients with CLL, twelve were SIgM+ and/or SIgD+, only four κ+ or λ+ and one had no SIg. The cells of the LSL and one of the HCL patients were SIgM+ and SIgD+, whilst the cells of the other HCL patient were SIgG, λ+. None of the other patients had more than 10{\%} SIgG+ cells. The ALL and Sezary syndrome patients had low numbers of SIg+ and Fc receptor positive cells. These data indicate that lymphocytes of patients with B-cell leukaemias can carry different classes of Fc receptors simultaneously; the different classes are found in a decreasing frequency of IgG > IgM > IgE.",
author = "Spiegelberg, {H. L.} and Dainer, {Paul M}",
year = "1979",
month = "1",
day = "1",
language = "English (US)",
volume = "35",
pages = "286--295",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Fc receptors for IgG, IgM and IgE on human leukaemic lymphocytes

AU - Spiegelberg, H. L.

AU - Dainer, Paul M

PY - 1979/1/1

Y1 - 1979/1/1

N2 - Fc receptors for IgG, IgM, IgE and the cell surface immunoglobulins (SIg) were analysed on lymphocytes from seventeen patients with chronic lymphatic leukaemia (CLL), one with lymphosarcoma cell leukaemia (LSL), two each with hairy cell leukaemia (HCL), acute lymphatic leukaemia (ALL) and Sezary syndrome. Fc receptors for IgG and IgM were detected by rosette formation with ox erythrocytes (E(O)) sensitized with rabbit IgG (E(O)A(G)) and IgM (E(O)A(M)) anti-E(O) antibodies, respectively. Fc receptors for IgE were analysed either with E(O) coated with glutaraldehyde coupled complexes consisting of rabbit Fab' fragments of anti-E(O) antibodies and Fc fragments of an IgE myeloma protein (E(O)A(E)), or with aldehyde fixed E(O) to which IgE was absorbed. SIg of classes IgM, IgD, IgG and κ and λ light chain were detected with E(O) coated with complexes consisting of Fab'-anti-E(O) and purified F(ab')2 fragments of specific goat antibodies. Lymphocytes of all patients with CLL, LSL and HCL had Fc receptors for IgG (65 ± 15% E(O)A(G)+, normal 22.0 ± 5.8%). Ten patients had significant numbers of cells with IgM Fc receptors (37 ± 22% E(O)A(M)+, normal 1.2 ± 1.5%) which were detected without overnight culturing of the lymphocytes. Lymphocytes of four patients (two CLL, one LSL, one HCL) had Fc receptors for IgE (22 - 88% E(O)A(E)+, normal 1.8 ± 0.7%). The cells of three of these four patients were also E(O)A(M)+. The high numbers of rosetting cells indicated that individual lymphocytes must have carried more than one class of Fc receptors. The lymphocytes of the ALL and Sezary syndrome patients had few Fc receptor positive cells. Of the seventeen patients with CLL, twelve were SIgM+ and/or SIgD+, only four κ+ or λ+ and one had no SIg. The cells of the LSL and one of the HCL patients were SIgM+ and SIgD+, whilst the cells of the other HCL patient were SIgG, λ+. None of the other patients had more than 10% SIgG+ cells. The ALL and Sezary syndrome patients had low numbers of SIg+ and Fc receptor positive cells. These data indicate that lymphocytes of patients with B-cell leukaemias can carry different classes of Fc receptors simultaneously; the different classes are found in a decreasing frequency of IgG > IgM > IgE.

AB - Fc receptors for IgG, IgM, IgE and the cell surface immunoglobulins (SIg) were analysed on lymphocytes from seventeen patients with chronic lymphatic leukaemia (CLL), one with lymphosarcoma cell leukaemia (LSL), two each with hairy cell leukaemia (HCL), acute lymphatic leukaemia (ALL) and Sezary syndrome. Fc receptors for IgG and IgM were detected by rosette formation with ox erythrocytes (E(O)) sensitized with rabbit IgG (E(O)A(G)) and IgM (E(O)A(M)) anti-E(O) antibodies, respectively. Fc receptors for IgE were analysed either with E(O) coated with glutaraldehyde coupled complexes consisting of rabbit Fab' fragments of anti-E(O) antibodies and Fc fragments of an IgE myeloma protein (E(O)A(E)), or with aldehyde fixed E(O) to which IgE was absorbed. SIg of classes IgM, IgD, IgG and κ and λ light chain were detected with E(O) coated with complexes consisting of Fab'-anti-E(O) and purified F(ab')2 fragments of specific goat antibodies. Lymphocytes of all patients with CLL, LSL and HCL had Fc receptors for IgG (65 ± 15% E(O)A(G)+, normal 22.0 ± 5.8%). Ten patients had significant numbers of cells with IgM Fc receptors (37 ± 22% E(O)A(M)+, normal 1.2 ± 1.5%) which were detected without overnight culturing of the lymphocytes. Lymphocytes of four patients (two CLL, one LSL, one HCL) had Fc receptors for IgE (22 - 88% E(O)A(E)+, normal 1.8 ± 0.7%). The cells of three of these four patients were also E(O)A(M)+. The high numbers of rosetting cells indicated that individual lymphocytes must have carried more than one class of Fc receptors. The lymphocytes of the ALL and Sezary syndrome patients had few Fc receptor positive cells. Of the seventeen patients with CLL, twelve were SIgM+ and/or SIgD+, only four κ+ or λ+ and one had no SIg. The cells of the LSL and one of the HCL patients were SIgM+ and SIgD+, whilst the cells of the other HCL patient were SIgG, λ+. None of the other patients had more than 10% SIgG+ cells. The ALL and Sezary syndrome patients had low numbers of SIg+ and Fc receptor positive cells. These data indicate that lymphocytes of patients with B-cell leukaemias can carry different classes of Fc receptors simultaneously; the different classes are found in a decreasing frequency of IgG > IgM > IgE.

UR - http://www.scopus.com/inward/record.url?scp=0018381976&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018381976&partnerID=8YFLogxK

M3 - Article

C2 - 312174

AN - SCOPUS:0018381976

VL - 35

SP - 286

EP - 295

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 2

ER -