Magnetic circular dichroism can detect the presence of certain special marker molecules in systems which contain overlapping absorption bands due to several other chromophores. The magnetic CD (MCD) of a marker molecule is relatively insensitive to the molecule's environment, in contrast to natural CD which may be due entirely to asymmetric molecular environments. To qualify as an MCD marker, a molecule must possess degenerate or nearly degenerate transitions. Thus MCD markers will often be excellent CD reporter groups also. The ultraviolet absorption bands of spinach ferredoxin illustrate these concepts.
ASJC Scopus subject areas