Fibrocystin/polyductin, found in the same protein complex with polycystin-2, regulates calcium responses in kidney epithelia

Shixuan Wang, Jingjing Zhang, Surya M. Nauli, Xiaogang Li, Patrick G. Starremans, Ying Luo, Kristina A. Roberts, Jing Zhou

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Recent evidence suggests that fibrocystin/polyductin (FPC), polycystin-1 (PC1), and polycystin-2 (PC2) are all localized at the plasma membrane and the primary cilium, where PC1 and PC2 contribute to fluid flow sensation and may function in the same mechanotransduction pathways. To further define the exact subcellular localization of FPC, the protein product encoded by the PKHD1 gene responsible for autosomal recessive polycystic kidney disease (PKD) in humans, and whether FPC has direct and/or indirect cross talk with PC2, which, in turn, is pivotal for the pathogenesis of autosomal dominant PKD, we performed double immunostaining and coimmimoprecipitation as well as a microfluorimetry study of kidney tubular epithelial cells. FPC and PC2 are found to completely or partially colocalize at the plasma membrane and the primary cilium and can be reciprocally coimmunoprecipitated. Although incomplete removal of FPC by small interfering RNA and antibody 803 to intracellular epitopes of FPC did not abolish flow-induced intracellular calcium responses, antibody 804 to extracellular epitopes of FPC blocked cellular calcium responses to flow stimulation. These findings suggest that FPC and polycystins share, at least in part, a common mechanotransduction pathway.

Original languageEnglish (US)
Pages (from-to)3241-3252
Number of pages12
JournalMolecular and Cellular Biology
Volume27
Issue number8
DOIs
StatePublished - Apr 1 2007

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Epithelium
Calcium
Kidney
Cilia
Epitopes
Proteins
Autosomal Recessive Polycystic Kidney
TRPP Cation Channels
Cell Membrane
Cytophotometry
Autosomal Dominant Polycystic Kidney
Small Interfering RNA
Antibody Formation
Epithelial Cells
polycystic kidney disease 2 protein
Antibodies
Genes
polycystic kidney disease 1 protein

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Fibrocystin/polyductin, found in the same protein complex with polycystin-2, regulates calcium responses in kidney epithelia. / Wang, Shixuan; Zhang, Jingjing; Nauli, Surya M.; Li, Xiaogang; Starremans, Patrick G.; Luo, Ying; Roberts, Kristina A.; Zhou, Jing.

In: Molecular and Cellular Biology, Vol. 27, No. 8, 01.04.2007, p. 3241-3252.

Research output: Contribution to journalArticle

Wang, Shixuan ; Zhang, Jingjing ; Nauli, Surya M. ; Li, Xiaogang ; Starremans, Patrick G. ; Luo, Ying ; Roberts, Kristina A. ; Zhou, Jing. / Fibrocystin/polyductin, found in the same protein complex with polycystin-2, regulates calcium responses in kidney epithelia. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 8. pp. 3241-3252.
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