Fibronectin induces matrix metalloproteinase-2 expression in ovarian cancer cells

Chunhong Yan, C. Li, F. Tian

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: To investigate the effects of extracelluar matrix proteins on matrix metalloproteinases (MMPs) expression by cancer cells, and the underlying mechanisms. METHODS: Following stimulation of SKOV3 ovarian cancer cells by fibronectin, MMPs secretion and cellular mRNA contents were assayed by gelatin-zymography and RT-PCR, respectively. The activity of MMP-2 promoter was monitored by cellular luciferase activity after the cells were transfected with MMP-2 promoter-luciferase construct. Cellular p53 contents were determined by Western blot analysis. RESULTS: Fibronectin (5 micrograms/ml) was found to stimulate the secretion of MMP-2 but not MMP-9 by SKOV3 cells. The stimulation was enhanced with the increase in fibronectin concentration. When SKOV3 cells were treated with 10 micrograms/ml fibronectin for 1 hr, the cellular MMP-2 mRNA dramatically increased. However, with increase in stimulation time, MMP-2 mRNA content decreased. Fibronectin induced an increase in luciferase activity in cells transfected with MMP-2 promoter construct, whilst curcumin (50 mumol/L), a potent transcription factor AP-1 inhibitor, could not block the fibronectin-induced increase in MMP-2 promoter activity. Fibronectin also induced an increase in p53 content of SKOV3 cells. CONCLUSION: Fibronectin stimulates ovarian cancer cells to secret MMP-2 via its enhancing effect on MMP-2 promoter activity with resultant increase in MMP-2 transcription. The effect might involve a pathway associated with p53 but independent of AP-1.

Original languageEnglish (US)
Pages (from-to)109-112
Number of pages4
JournalZhonghua zhong liu za zhi [Chinese journal of oncology]
Volume22
Issue number2
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 2
Fibronectins
Ovarian Neoplasms
Luciferases
Transcription Factor AP-1
Matrix Metalloproteinases
Messenger RNA
Curcumin
Matrix Metalloproteinase 9
Gelatin
Western Blotting
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fibronectin induces matrix metalloproteinase-2 expression in ovarian cancer cells. / Yan, Chunhong; Li, C.; Tian, F.

In: Zhonghua zhong liu za zhi [Chinese journal of oncology], Vol. 22, No. 2, 01.01.2000, p. 109-112.

Research output: Contribution to journalArticle

@article{26bdfe4275524cc1bc268e6a89237b4f,
title = "Fibronectin induces matrix metalloproteinase-2 expression in ovarian cancer cells",
abstract = "OBJECTIVE: To investigate the effects of extracelluar matrix proteins on matrix metalloproteinases (MMPs) expression by cancer cells, and the underlying mechanisms. METHODS: Following stimulation of SKOV3 ovarian cancer cells by fibronectin, MMPs secretion and cellular mRNA contents were assayed by gelatin-zymography and RT-PCR, respectively. The activity of MMP-2 promoter was monitored by cellular luciferase activity after the cells were transfected with MMP-2 promoter-luciferase construct. Cellular p53 contents were determined by Western blot analysis. RESULTS: Fibronectin (5 micrograms/ml) was found to stimulate the secretion of MMP-2 but not MMP-9 by SKOV3 cells. The stimulation was enhanced with the increase in fibronectin concentration. When SKOV3 cells were treated with 10 micrograms/ml fibronectin for 1 hr, the cellular MMP-2 mRNA dramatically increased. However, with increase in stimulation time, MMP-2 mRNA content decreased. Fibronectin induced an increase in luciferase activity in cells transfected with MMP-2 promoter construct, whilst curcumin (50 mumol/L), a potent transcription factor AP-1 inhibitor, could not block the fibronectin-induced increase in MMP-2 promoter activity. Fibronectin also induced an increase in p53 content of SKOV3 cells. CONCLUSION: Fibronectin stimulates ovarian cancer cells to secret MMP-2 via its enhancing effect on MMP-2 promoter activity with resultant increase in MMP-2 transcription. The effect might involve a pathway associated with p53 but independent of AP-1.",
author = "Chunhong Yan and C. Li and F. Tian",
year = "2000",
month = "1",
day = "1",
language = "English (US)",
volume = "22",
pages = "109--112",
journal = "Chinese Journal of Oncology",
issn = "0253-3766",
publisher = "Chinese Academy of Medical Sciences",
number = "2",

}

TY - JOUR

T1 - Fibronectin induces matrix metalloproteinase-2 expression in ovarian cancer cells

AU - Yan, Chunhong

AU - Li, C.

AU - Tian, F.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - OBJECTIVE: To investigate the effects of extracelluar matrix proteins on matrix metalloproteinases (MMPs) expression by cancer cells, and the underlying mechanisms. METHODS: Following stimulation of SKOV3 ovarian cancer cells by fibronectin, MMPs secretion and cellular mRNA contents were assayed by gelatin-zymography and RT-PCR, respectively. The activity of MMP-2 promoter was monitored by cellular luciferase activity after the cells were transfected with MMP-2 promoter-luciferase construct. Cellular p53 contents were determined by Western blot analysis. RESULTS: Fibronectin (5 micrograms/ml) was found to stimulate the secretion of MMP-2 but not MMP-9 by SKOV3 cells. The stimulation was enhanced with the increase in fibronectin concentration. When SKOV3 cells were treated with 10 micrograms/ml fibronectin for 1 hr, the cellular MMP-2 mRNA dramatically increased. However, with increase in stimulation time, MMP-2 mRNA content decreased. Fibronectin induced an increase in luciferase activity in cells transfected with MMP-2 promoter construct, whilst curcumin (50 mumol/L), a potent transcription factor AP-1 inhibitor, could not block the fibronectin-induced increase in MMP-2 promoter activity. Fibronectin also induced an increase in p53 content of SKOV3 cells. CONCLUSION: Fibronectin stimulates ovarian cancer cells to secret MMP-2 via its enhancing effect on MMP-2 promoter activity with resultant increase in MMP-2 transcription. The effect might involve a pathway associated with p53 but independent of AP-1.

AB - OBJECTIVE: To investigate the effects of extracelluar matrix proteins on matrix metalloproteinases (MMPs) expression by cancer cells, and the underlying mechanisms. METHODS: Following stimulation of SKOV3 ovarian cancer cells by fibronectin, MMPs secretion and cellular mRNA contents were assayed by gelatin-zymography and RT-PCR, respectively. The activity of MMP-2 promoter was monitored by cellular luciferase activity after the cells were transfected with MMP-2 promoter-luciferase construct. Cellular p53 contents were determined by Western blot analysis. RESULTS: Fibronectin (5 micrograms/ml) was found to stimulate the secretion of MMP-2 but not MMP-9 by SKOV3 cells. The stimulation was enhanced with the increase in fibronectin concentration. When SKOV3 cells were treated with 10 micrograms/ml fibronectin for 1 hr, the cellular MMP-2 mRNA dramatically increased. However, with increase in stimulation time, MMP-2 mRNA content decreased. Fibronectin induced an increase in luciferase activity in cells transfected with MMP-2 promoter construct, whilst curcumin (50 mumol/L), a potent transcription factor AP-1 inhibitor, could not block the fibronectin-induced increase in MMP-2 promoter activity. Fibronectin also induced an increase in p53 content of SKOV3 cells. CONCLUSION: Fibronectin stimulates ovarian cancer cells to secret MMP-2 via its enhancing effect on MMP-2 promoter activity with resultant increase in MMP-2 transcription. The effect might involve a pathway associated with p53 but independent of AP-1.

UR - http://www.scopus.com/inward/record.url?scp=0034160565&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034160565&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 109

EP - 112

JO - Chinese Journal of Oncology

JF - Chinese Journal of Oncology

SN - 0253-3766

IS - 2

ER -