Fibulin-1 is required for morphogenesis of neural crest-derived structures

Marion Anne Cooley, Christine B. Kern, Victor M. Fresco, Andy Wessels, Robert P. Thompson, Tim C. McQuinn, Waleed O. Twal, Corey H. Mjaatvedt, Christopher J. Drake, W. Scott Argraves

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Here we report that mouse embryos homozygous for a gene trap insertion in the fibulin-1 (Fbln1) gene are deficient in Fbln1 and exhibit cardiac ventricular wall thinning and ventricular septal defects with double outlet right ventricle or overriding aorta. Fbln1 nulls also display anomalies of aortic arch arteries, hypoplasia of the thymus and thyroid, underdeveloped skull bones, malformations of cranial nerves and hemorrhagic blood vessels in the head and neck. The spectrum of malformations is consistent with Fbln1 influencing neural crest cell (NCC)-dependent development of these tissues. This is supported by evidence that Fbln1 expression is associated with streams of cranial NCCs migrating adjacent to rhombomeres 2-7 and that Fbln1-deficient embryos display patterning anomalies of NCCs forming cranial nerves IX and X, which derive from rhombomeres 6 and 7. Additionally, Fbln1-deficient embryos show increased apoptosis in areas populated by NCCs derived from rhombomeres 4, 6 and 7. Based on these findings, it is concluded that Fbln1 is required for the directed migration and survival of cranial NCCs contributing to the development of pharyngeal glands, craniofacial skeleton, cranial nerves, aortic arch arteries, cardiac outflow tract and cephalic blood vessels.

Original languageEnglish (US)
Pages (from-to)336-345
Number of pages10
JournalDevelopmental Biology
Volume319
Issue number2
DOIs
StatePublished - Jul 15 2008
Externally publishedYes

Fingerprint

Neural Crest
Morphogenesis
Embryonic Structures
Cranial Nerves
Thoracic Aorta
Blood Vessels
Arteries
Head
Thyroid Dysgenesis
Double Outlet Right Ventricle
Glossopharyngeal Nerve
Vagus Nerve
Insertional Mutagenesis
Ventricular Heart Septal Defects
fibulin
Skull
Skeleton
Thymus Gland
Aorta
Neck

Keywords

  • 22q11.2 deletion
  • Anterior heart field
  • Bleeding
  • Cardiac abnormalities
  • Cranial nerves
  • DiGeorge syndrome
  • Extracellular matrix
  • Fibulin
  • Gene trap
  • Heart development
  • Knockout
  • Motility
  • Neural crest
  • Outflow tract
  • Secondary heart field

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Cooley, M. A., Kern, C. B., Fresco, V. M., Wessels, A., Thompson, R. P., McQuinn, T. C., ... Argraves, W. S. (2008). Fibulin-1 is required for morphogenesis of neural crest-derived structures. Developmental Biology, 319(2), 336-345. https://doi.org/10.1016/j.ydbio.2008.04.029

Fibulin-1 is required for morphogenesis of neural crest-derived structures. / Cooley, Marion Anne; Kern, Christine B.; Fresco, Victor M.; Wessels, Andy; Thompson, Robert P.; McQuinn, Tim C.; Twal, Waleed O.; Mjaatvedt, Corey H.; Drake, Christopher J.; Argraves, W. Scott.

In: Developmental Biology, Vol. 319, No. 2, 15.07.2008, p. 336-345.

Research output: Contribution to journalArticle

Cooley, MA, Kern, CB, Fresco, VM, Wessels, A, Thompson, RP, McQuinn, TC, Twal, WO, Mjaatvedt, CH, Drake, CJ & Argraves, WS 2008, 'Fibulin-1 is required for morphogenesis of neural crest-derived structures', Developmental Biology, vol. 319, no. 2, pp. 336-345. https://doi.org/10.1016/j.ydbio.2008.04.029
Cooley, Marion Anne ; Kern, Christine B. ; Fresco, Victor M. ; Wessels, Andy ; Thompson, Robert P. ; McQuinn, Tim C. ; Twal, Waleed O. ; Mjaatvedt, Corey H. ; Drake, Christopher J. ; Argraves, W. Scott. / Fibulin-1 is required for morphogenesis of neural crest-derived structures. In: Developmental Biology. 2008 ; Vol. 319, No. 2. pp. 336-345.
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