Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues

Awtar Krishan, Avi Oppenheimer, Wei You, Robert Dubbin, Deepa Sharma, Balakrishna L Lokeshwar

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined.

Original languageEnglish (US)
Pages (from-to)1922-1930
Number of pages9
JournalClinical Cancer Research
Volume6
Issue number5
StatePublished - May 1 2000

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Androgen Receptors
Prostate
Neoplasms
Aneuploidy
Genetic Markers
Paraffin
Formaldehyde
Lasers
Cell Line
Growth
Therapeutics

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues. / Krishan, Awtar; Oppenheimer, Avi; You, Wei; Dubbin, Robert; Sharma, Deepa; Lokeshwar, Balakrishna L.

In: Clinical Cancer Research, Vol. 6, No. 5, 01.05.2000, p. 1922-1930.

Research output: Contribution to journalArticle

Krishan, A, Oppenheimer, A, You, W, Dubbin, R, Sharma, D & Lokeshwar, BL 2000, 'Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues', Clinical Cancer Research, vol. 6, no. 5, pp. 1922-1930.
Krishan, Awtar ; Oppenheimer, Avi ; You, Wei ; Dubbin, Robert ; Sharma, Deepa ; Lokeshwar, Balakrishna L. / Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues. In: Clinical Cancer Research. 2000 ; Vol. 6, No. 5. pp. 1922-1930.
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