Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues

Awtar Krishan, Avi Oppenheimer, Wei You, Robert Dubbin, Deepa Sharma, Balakrishna L. Lokeshwar

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined.

Original languageEnglish (US)
Pages (from-to)1922-1930
Number of pages9
JournalClinical Cancer Research
Volume6
Issue number5
Publication statusPublished - May 1 2000
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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