TY - JOUR
T1 - Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues
AU - Krishan, Awtar
AU - Oppenheimer, Avi
AU - You, Wei
AU - Dubbin, Robert
AU - Sharma, Deepa
AU - Lokeshwar, Balakrishna L.
PY - 2000/5
Y1 - 2000/5
N2 - Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined.
AB - Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined.
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M3 - Article
C2 - 10815917
AN - SCOPUS:0034109297
SN - 1078-0432
VL - 6
SP - 1922
EP - 1930
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 5
ER -
