Flow cytometric immunophenotypic assessment of T-cell clonality by V β repertoire analysis: Detection of T-cell clonality at diagnosis and monitoring of minimal residual disease following therapy

Prashant Tembhare, Constance M. Yuan, Liqiang Xi, John C. Morris, David Liewehr, David Venzon, John Edward Janik, Mark Raffeld, Maryalice Stetler-Stevenson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Flow cytometric T-cell receptor (TCR)-Vβ repertoire analysis (TCR-Vβ-R) is a sensitive method for detection of T-cell clonality; however, no uniform approach exists to define clonality in neoplastic T cells. TCR-Vβ-R was evaluated in patients with a diagnosis of T-cell neoplasia in initial diagnostic specimens from 41 patients and for minimal residual disease (MRD) monitoring in 61 sequential samples from 14 patients with mature T-cell neoplasia. Gating strategies and criteria for detection of T-cell clonality were determined. In all 41 initial specimens, T-cell clonality was demonstrated via TCR-Vβ-R. The frequency of Vβ usage was consistent with random neoplastic transformation of TCR-Vβ subsets. MRD was successfully detected in follow-up samples from all 14 patients evaluated, Furthermore, MRD after therapy was quantitated in 48 peripheral blood specimens. TCR-Vβ-R analysis is a sensitive method for detection of T-cell clonality and is useful for diagnosis and MRD detection in multiple specimen types.

Original languageEnglish (US)
Pages (from-to)890-900
Number of pages11
JournalAmerican Journal of Clinical Pathology
Volume135
Issue number6
DOIs
StatePublished - Jun 1 2011

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Residual Neoplasm
T-Cell Antigen Receptor
T-Lymphocytes
Therapeutics
Neoplasms

Keywords

  • Flow cytometry
  • T-cell clonality
  • V repertoire

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Flow cytometric immunophenotypic assessment of T-cell clonality by V β repertoire analysis : Detection of T-cell clonality at diagnosis and monitoring of minimal residual disease following therapy. / Tembhare, Prashant; Yuan, Constance M.; Xi, Liqiang; Morris, John C.; Liewehr, David; Venzon, David; Janik, John Edward; Raffeld, Mark; Stetler-Stevenson, Maryalice.

In: American Journal of Clinical Pathology, Vol. 135, No. 6, 01.06.2011, p. 890-900.

Research output: Contribution to journalArticle

Tembhare, Prashant ; Yuan, Constance M. ; Xi, Liqiang ; Morris, John C. ; Liewehr, David ; Venzon, David ; Janik, John Edward ; Raffeld, Mark ; Stetler-Stevenson, Maryalice. / Flow cytometric immunophenotypic assessment of T-cell clonality by V β repertoire analysis : Detection of T-cell clonality at diagnosis and monitoring of minimal residual disease following therapy. In: American Journal of Clinical Pathology. 2011 ; Vol. 135, No. 6. pp. 890-900.
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