Flow cytometric immunophenotypic profiles of mature gamma delta T-cell malignancies involving peripheral blood and bone marrow

Ejaz Ahmad, Douglas W. Kingma, Elaine S. Jaffe, Jeffrey A. Schrager, John Janik, Wyndham Wilson, Maryalice Stetler-Stevenson

Research output: Contribution to journalReview article

40 Citations (Scopus)

Abstract

Background: In this study we compared clinical findings with flow cytometric immunophenotypic results in a series of patients with aggressive and indolent gamma delta T-cell malignancies with peripheral blood and/or bone marrow involvement. Methods: Gamma delta T-cell malignancies were detected based on flow cytometric demonstration of an abnormal T-cell population staining positive with T-cell receptor gamma delta and confirmed by morphologic and clinical reviews. Clinical data were obtained through chart review and discussion with the patients' physicians. Results: Blood or bone marrow involvement was present in all patients. Hepatosplenic and cutaneous gamma delta T-cell lymphomas had an aggressive clinical course, whereas the gamma delta T-cell large granular lymphocyte (LGL) leukemias had an indolent course. Expressions of CD5, CD8, CD16, and CD57 differed in gamma delta T-cell LGL leukemia compared with hepatosplenic and cutaneous gamma delta T-cell lymphomas. Conclusions: Gamma delta T-cell malignancies have a poor prognosis with the exception of gamma delta T-cell LGL leukemia (indolent process). Because CD57 expression is specific for gamma delta T-cell LGL leukemias, expression of this antigen may be associated with a more indolent clinical course. Because cutaneous gamma delta T-cell lymphoma can present with peripheral blood involvement, flow cytometric evaluation of peripheral blood is important in staging these patients.

Original languageEnglish (US)
Pages (from-to)6-12
Number of pages7
JournalCytometry Part B - Clinical Cytometry
Volume67
Issue number1
DOIs
StatePublished - Sep 1 2005

Fingerprint

Somatostatin-Secreting Cells
Bone Marrow
T-Lymphocytes
Large Granular Lymphocytic Leukemia
Neoplasms
T-Cell Lymphoma
Skin
Antigen Receptors, T-Cell, gamma-delta
Staining and Labeling
Physicians
Antigens

Keywords

  • Flow cytometry
  • Gamma delta T cell
  • Large granular lymphocyte leukemia
  • Lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Flow cytometric immunophenotypic profiles of mature gamma delta T-cell malignancies involving peripheral blood and bone marrow. / Ahmad, Ejaz; Kingma, Douglas W.; Jaffe, Elaine S.; Schrager, Jeffrey A.; Janik, John; Wilson, Wyndham; Stetler-Stevenson, Maryalice.

In: Cytometry Part B - Clinical Cytometry, Vol. 67, No. 1, 01.09.2005, p. 6-12.

Research output: Contribution to journalReview article

Ahmad, Ejaz ; Kingma, Douglas W. ; Jaffe, Elaine S. ; Schrager, Jeffrey A. ; Janik, John ; Wilson, Wyndham ; Stetler-Stevenson, Maryalice. / Flow cytometric immunophenotypic profiles of mature gamma delta T-cell malignancies involving peripheral blood and bone marrow. In: Cytometry Part B - Clinical Cytometry. 2005 ; Vol. 67, No. 1. pp. 6-12.
@article{8655ddccf6344df797534cbb458523da,
title = "Flow cytometric immunophenotypic profiles of mature gamma delta T-cell malignancies involving peripheral blood and bone marrow",
abstract = "Background: In this study we compared clinical findings with flow cytometric immunophenotypic results in a series of patients with aggressive and indolent gamma delta T-cell malignancies with peripheral blood and/or bone marrow involvement. Methods: Gamma delta T-cell malignancies were detected based on flow cytometric demonstration of an abnormal T-cell population staining positive with T-cell receptor gamma delta and confirmed by morphologic and clinical reviews. Clinical data were obtained through chart review and discussion with the patients' physicians. Results: Blood or bone marrow involvement was present in all patients. Hepatosplenic and cutaneous gamma delta T-cell lymphomas had an aggressive clinical course, whereas the gamma delta T-cell large granular lymphocyte (LGL) leukemias had an indolent course. Expressions of CD5, CD8, CD16, and CD57 differed in gamma delta T-cell LGL leukemia compared with hepatosplenic and cutaneous gamma delta T-cell lymphomas. Conclusions: Gamma delta T-cell malignancies have a poor prognosis with the exception of gamma delta T-cell LGL leukemia (indolent process). Because CD57 expression is specific for gamma delta T-cell LGL leukemias, expression of this antigen may be associated with a more indolent clinical course. Because cutaneous gamma delta T-cell lymphoma can present with peripheral blood involvement, flow cytometric evaluation of peripheral blood is important in staging these patients.",
keywords = "Flow cytometry, Gamma delta T cell, Large granular lymphocyte leukemia, Lymphoma",
author = "Ejaz Ahmad and Kingma, {Douglas W.} and Jaffe, {Elaine S.} and Schrager, {Jeffrey A.} and John Janik and Wyndham Wilson and Maryalice Stetler-Stevenson",
year = "2005",
month = "9",
day = "1",
doi = "10.1002/cyto.b.20063",
language = "English (US)",
volume = "67",
pages = "6--12",
journal = "Cytometry",
issn = "0196-4763",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Flow cytometric immunophenotypic profiles of mature gamma delta T-cell malignancies involving peripheral blood and bone marrow

AU - Ahmad, Ejaz

AU - Kingma, Douglas W.

AU - Jaffe, Elaine S.

AU - Schrager, Jeffrey A.

AU - Janik, John

AU - Wilson, Wyndham

AU - Stetler-Stevenson, Maryalice

PY - 2005/9/1

Y1 - 2005/9/1

N2 - Background: In this study we compared clinical findings with flow cytometric immunophenotypic results in a series of patients with aggressive and indolent gamma delta T-cell malignancies with peripheral blood and/or bone marrow involvement. Methods: Gamma delta T-cell malignancies were detected based on flow cytometric demonstration of an abnormal T-cell population staining positive with T-cell receptor gamma delta and confirmed by morphologic and clinical reviews. Clinical data were obtained through chart review and discussion with the patients' physicians. Results: Blood or bone marrow involvement was present in all patients. Hepatosplenic and cutaneous gamma delta T-cell lymphomas had an aggressive clinical course, whereas the gamma delta T-cell large granular lymphocyte (LGL) leukemias had an indolent course. Expressions of CD5, CD8, CD16, and CD57 differed in gamma delta T-cell LGL leukemia compared with hepatosplenic and cutaneous gamma delta T-cell lymphomas. Conclusions: Gamma delta T-cell malignancies have a poor prognosis with the exception of gamma delta T-cell LGL leukemia (indolent process). Because CD57 expression is specific for gamma delta T-cell LGL leukemias, expression of this antigen may be associated with a more indolent clinical course. Because cutaneous gamma delta T-cell lymphoma can present with peripheral blood involvement, flow cytometric evaluation of peripheral blood is important in staging these patients.

AB - Background: In this study we compared clinical findings with flow cytometric immunophenotypic results in a series of patients with aggressive and indolent gamma delta T-cell malignancies with peripheral blood and/or bone marrow involvement. Methods: Gamma delta T-cell malignancies were detected based on flow cytometric demonstration of an abnormal T-cell population staining positive with T-cell receptor gamma delta and confirmed by morphologic and clinical reviews. Clinical data were obtained through chart review and discussion with the patients' physicians. Results: Blood or bone marrow involvement was present in all patients. Hepatosplenic and cutaneous gamma delta T-cell lymphomas had an aggressive clinical course, whereas the gamma delta T-cell large granular lymphocyte (LGL) leukemias had an indolent course. Expressions of CD5, CD8, CD16, and CD57 differed in gamma delta T-cell LGL leukemia compared with hepatosplenic and cutaneous gamma delta T-cell lymphomas. Conclusions: Gamma delta T-cell malignancies have a poor prognosis with the exception of gamma delta T-cell LGL leukemia (indolent process). Because CD57 expression is specific for gamma delta T-cell LGL leukemias, expression of this antigen may be associated with a more indolent clinical course. Because cutaneous gamma delta T-cell lymphoma can present with peripheral blood involvement, flow cytometric evaluation of peripheral blood is important in staging these patients.

KW - Flow cytometry

KW - Gamma delta T cell

KW - Large granular lymphocyte leukemia

KW - Lymphoma

UR - http://www.scopus.com/inward/record.url?scp=24744468615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24744468615&partnerID=8YFLogxK

U2 - 10.1002/cyto.b.20063

DO - 10.1002/cyto.b.20063

M3 - Review article

C2 - 15973700

AN - SCOPUS:24744468615

VL - 67

SP - 6

EP - 12

JO - Cytometry

JF - Cytometry

SN - 0196-4763

IS - 1

ER -