Fluoride Reabsorption by Nonionic Diffusion in the Distal Nephron of the Dog

Gary M. Whitford, David Henry Pashley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This study was done to test the hypothesis that fluoride reabsorption is extensive from the distal nephron, the major site for tubular fluid acidification, and to compare the distal nephron handling of fluoride and chloride. Ten stop-flow studies were done in five dogs anesthetized with pentobarbital. Urinary alkalinization was achieved by the intravenous infusion of sodium bicarbonate and acetazolamide or lithium chloride. Acidification was achieved by the infusion of sodium nitrate or sodium sulfate. The results indicate that the extent of fluoride reabsorption from the distal nephron is inversely correlated with urinary pH (P < 0.001). When the urine was strongly acidified by the infusion of sodium sulfate, urine to plasma fluoride concentration ratios were less than 1.0, a finding not previously reported from studies of the renal handling of fluoride. The reabsorption of fluoride from the distal nephron was not correlated consistently with that of chloride. The results indicate that the distal nephron is an important site for the reabsorption of fluoride and they provide additional evidence that HF is the permeating moiety.

Original languageEnglish (US)
Pages (from-to)178-183
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume196
Issue number2
DOIs
StatePublished - Jan 1 1991

Fingerprint

Nephrons
Fluorides
Dogs
Acidification
Chlorides
Urine
Lithium Chloride
Acetazolamide
Sodium Bicarbonate
Pentobarbital
Intravenous Infusions
Kidney
Plasmas
Fluids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fluoride Reabsorption by Nonionic Diffusion in the Distal Nephron of the Dog. / Whitford, Gary M.; Pashley, David Henry.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 196, No. 2, 01.01.1991, p. 178-183.

Research output: Contribution to journalArticle

@article{fe29066fbb9d44289f0460e0a097cb76,
title = "Fluoride Reabsorption by Nonionic Diffusion in the Distal Nephron of the Dog",
abstract = "This study was done to test the hypothesis that fluoride reabsorption is extensive from the distal nephron, the major site for tubular fluid acidification, and to compare the distal nephron handling of fluoride and chloride. Ten stop-flow studies were done in five dogs anesthetized with pentobarbital. Urinary alkalinization was achieved by the intravenous infusion of sodium bicarbonate and acetazolamide or lithium chloride. Acidification was achieved by the infusion of sodium nitrate or sodium sulfate. The results indicate that the extent of fluoride reabsorption from the distal nephron is inversely correlated with urinary pH (P < 0.001). When the urine was strongly acidified by the infusion of sodium sulfate, urine to plasma fluoride concentration ratios were less than 1.0, a finding not previously reported from studies of the renal handling of fluoride. The reabsorption of fluoride from the distal nephron was not correlated consistently with that of chloride. The results indicate that the distal nephron is an important site for the reabsorption of fluoride and they provide additional evidence that HF is the permeating moiety.",
author = "Whitford, {Gary M.} and Pashley, {David Henry}",
year = "1991",
month = "1",
day = "1",
doi = "10.3181/00379727-196-43176",
language = "English (US)",
volume = "196",
pages = "178--183",
journal = "Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)",
issn = "1535-3702",
publisher = "Society for Experimental Biology and Medicine",
number = "2",

}

TY - JOUR

T1 - Fluoride Reabsorption by Nonionic Diffusion in the Distal Nephron of the Dog

AU - Whitford, Gary M.

AU - Pashley, David Henry

PY - 1991/1/1

Y1 - 1991/1/1

N2 - This study was done to test the hypothesis that fluoride reabsorption is extensive from the distal nephron, the major site for tubular fluid acidification, and to compare the distal nephron handling of fluoride and chloride. Ten stop-flow studies were done in five dogs anesthetized with pentobarbital. Urinary alkalinization was achieved by the intravenous infusion of sodium bicarbonate and acetazolamide or lithium chloride. Acidification was achieved by the infusion of sodium nitrate or sodium sulfate. The results indicate that the extent of fluoride reabsorption from the distal nephron is inversely correlated with urinary pH (P < 0.001). When the urine was strongly acidified by the infusion of sodium sulfate, urine to plasma fluoride concentration ratios were less than 1.0, a finding not previously reported from studies of the renal handling of fluoride. The reabsorption of fluoride from the distal nephron was not correlated consistently with that of chloride. The results indicate that the distal nephron is an important site for the reabsorption of fluoride and they provide additional evidence that HF is the permeating moiety.

AB - This study was done to test the hypothesis that fluoride reabsorption is extensive from the distal nephron, the major site for tubular fluid acidification, and to compare the distal nephron handling of fluoride and chloride. Ten stop-flow studies were done in five dogs anesthetized with pentobarbital. Urinary alkalinization was achieved by the intravenous infusion of sodium bicarbonate and acetazolamide or lithium chloride. Acidification was achieved by the infusion of sodium nitrate or sodium sulfate. The results indicate that the extent of fluoride reabsorption from the distal nephron is inversely correlated with urinary pH (P < 0.001). When the urine was strongly acidified by the infusion of sodium sulfate, urine to plasma fluoride concentration ratios were less than 1.0, a finding not previously reported from studies of the renal handling of fluoride. The reabsorption of fluoride from the distal nephron was not correlated consistently with that of chloride. The results indicate that the distal nephron is an important site for the reabsorption of fluoride and they provide additional evidence that HF is the permeating moiety.

UR - http://www.scopus.com/inward/record.url?scp=0026013585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026013585&partnerID=8YFLogxK

U2 - 10.3181/00379727-196-43176

DO - 10.3181/00379727-196-43176

M3 - Article

VL - 196

SP - 178

EP - 183

JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

SN - 1535-3702

IS - 2

ER -