Fluoride uptake by Streptococcus mutans 6715

Gary M. Whitford, G. S. Schuster, D. H. Pashley, P. Venkateswarlu

Research output: Contribution to journalArticle

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Abstract

The short-term kinetics of fluoride uptake by cells from 20- to 22-h cultures of S. mutans strain 6715 were studied using rapid filtration and centrifugation techniques. Saline-suspended organisms were diluted with fluoridecontaining solutions buffered at four different pH values (2.0, 4.0, 5.5, and 8.2). Fluoride disappearance from the medium was inversely related to pH and to the duration of the exposure at any given pH. The uptake was rapid and extensive at the lower pH values and decreased as the pH increased. Media fluoride concentrations subsequently increased; i.e., fluoride was released from the cells. The presence of glucose, cyanide, or iodoacetate did not influence the results. However, preincubation of the cells in fluoride-free buffers, followed by the addition of fluoride, reduced fluoride uptake markedly. Cell-to-media pH gradients were determined by the distribution of 14C-labeled 5,5-dimethyl-2,4-oxazolidinedione. Fluoride uptake was found to be a function of the magnitude of the pH gradient (P < 0.001). It is hypothesized that fluoride uptake occurs by the diffusion of hydrogen fluoride and the subsequent trapping of ionic fluoride.

Original languageEnglish (US)
Pages (from-to)680-687
Number of pages8
JournalInfection and Immunity
Volume18
Issue number3
StatePublished - Dec 1 1977

Fingerprint

Streptococcus mutans
Fluorides
Proton-Motive Force
Dimethadione
Hydrofluoric Acid
Iodoacetates
Cyanides
Centrifugation
Buffers
Glucose

ASJC Scopus subject areas

  • Immunology

Cite this

Whitford, G. M., Schuster, G. S., Pashley, D. H., & Venkateswarlu, P. (1977). Fluoride uptake by Streptococcus mutans 6715. Infection and Immunity, 18(3), 680-687.

Fluoride uptake by Streptococcus mutans 6715. / Whitford, Gary M.; Schuster, G. S.; Pashley, D. H.; Venkateswarlu, P.

In: Infection and Immunity, Vol. 18, No. 3, 01.12.1977, p. 680-687.

Research output: Contribution to journalArticle

Whitford, GM, Schuster, GS, Pashley, DH & Venkateswarlu, P 1977, 'Fluoride uptake by Streptococcus mutans 6715', Infection and Immunity, vol. 18, no. 3, pp. 680-687.
Whitford GM, Schuster GS, Pashley DH, Venkateswarlu P. Fluoride uptake by Streptococcus mutans 6715. Infection and Immunity. 1977 Dec 1;18(3):680-687.
Whitford, Gary M. ; Schuster, G. S. ; Pashley, D. H. ; Venkateswarlu, P. / Fluoride uptake by Streptococcus mutans 6715. In: Infection and Immunity. 1977 ; Vol. 18, No. 3. pp. 680-687.
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