Focal adhesion kinase in netrin-1 signaling

Xiu Rong Ren, Guo Li Ming, Yi Xie, Yan Hong, Dong Mei Sun, Zhong Qiu Zhao, Zhu Feng, Qiang Wang, Sangwoo Shim, Zhou Feng Chen, Hong Jun Song, Lin Mei, Wencheng Xiong

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Netrins are a family of secreted molecules that are important for axonal outgrowth and guidance in the developing nervous system. However, the signaling mechanisms that lie immediately downstream of netrin receptors remain poorly understood. Here we report that the netrin receptor DCC (deleted in colorectal cancer) interacts with the focal adhesion kinase (FAK), a kinase implicated in regulating cell adhesion and migration. FAK was expressed in developing brains and was localized with DCC in cultured neurons. Netrin-1 induced FAK and DCC tyrosine phosphorylation. Disruption of FAK signaling abolished netrin-1-induced neurite outgrowth and attractive growth cone turning. Taken together, these results indicate a new signaling mechanism for DCC, in which FAK is activated upon netrin-1 stimulation and mediates netrin-1 function; they also identify a critical role for FAK in axon navigation.

Original languageEnglish (US)
Pages (from-to)1204-1212
Number of pages9
JournalNature Neuroscience
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2004

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Focal Adhesion Protein-Tyrosine Kinases
Colorectal Neoplasms
Growth Cones
Cell Adhesion
Nervous System
Cell Movement
Axons
Tyrosine
netrin-1
Phosphotransferases
Phosphorylation
Neurons
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ren, X. R., Ming, G. L., Xie, Y., Hong, Y., Sun, D. M., Zhao, Z. Q., ... Xiong, W. (2004). Focal adhesion kinase in netrin-1 signaling. Nature Neuroscience, 7(11), 1204-1212. https://doi.org/10.1038/nn1330

Focal adhesion kinase in netrin-1 signaling. / Ren, Xiu Rong; Ming, Guo Li; Xie, Yi; Hong, Yan; Sun, Dong Mei; Zhao, Zhong Qiu; Feng, Zhu; Wang, Qiang; Shim, Sangwoo; Chen, Zhou Feng; Song, Hong Jun; Mei, Lin; Xiong, Wencheng.

In: Nature Neuroscience, Vol. 7, No. 11, 01.11.2004, p. 1204-1212.

Research output: Contribution to journalArticle

Ren, XR, Ming, GL, Xie, Y, Hong, Y, Sun, DM, Zhao, ZQ, Feng, Z, Wang, Q, Shim, S, Chen, ZF, Song, HJ, Mei, L & Xiong, W 2004, 'Focal adhesion kinase in netrin-1 signaling', Nature Neuroscience, vol. 7, no. 11, pp. 1204-1212. https://doi.org/10.1038/nn1330
Ren XR, Ming GL, Xie Y, Hong Y, Sun DM, Zhao ZQ et al. Focal adhesion kinase in netrin-1 signaling. Nature Neuroscience. 2004 Nov 1;7(11):1204-1212. https://doi.org/10.1038/nn1330
Ren, Xiu Rong ; Ming, Guo Li ; Xie, Yi ; Hong, Yan ; Sun, Dong Mei ; Zhao, Zhong Qiu ; Feng, Zhu ; Wang, Qiang ; Shim, Sangwoo ; Chen, Zhou Feng ; Song, Hong Jun ; Mei, Lin ; Xiong, Wencheng. / Focal adhesion kinase in netrin-1 signaling. In: Nature Neuroscience. 2004 ; Vol. 7, No. 11. pp. 1204-1212.
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