Folic acid-decorated polyamidoamine dendrimer mediates selective uptake and high expression of genes in head and neck cancer cells

Leyuan Xu, Shannon Kittrell, W. Andrew Yeudall, Hu Yang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aim: Folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) was synthesized and studied for targeted delivery of genes to head and neck cancer cells expressing high levels of folate receptors (FRs). Methods: Cellular uptake, targeting specificity, cytocompatibility and transfection efficiency were evaluated. Results: G4-FA competes with free FA for the same binding site. G4-FA facilitates the cellular uptake of DNA plasmids in a FR-dependent manner and selectively delivers plasmids to FR-high cells, leading to enhanced gene expression. Conclusion: G4-FA is a suitable vector to deliver genes selectively to head and neck cancer cells. The fundamental understandings of G4-FA as a vector and its encouraging transfection results for head and neck cancer cells provided support for its further testing in vivo.

Original languageEnglish (US)
Pages (from-to)2959-2973
Number of pages15
JournalNanomedicine
Volume11
Issue number22
DOIs
StatePublished - Nov 2016

Fingerprint

Dendrimers
Head and Neck Neoplasms
Folic Acid
cancer
Genes
Cells
Gene Expression
Acids
gene
acid
plasmid
efficiency
Transfection
Plasmids
Binding sites
Poly(amidoamine)
Gene expression
targeting
gene expression
DNA

Keywords

  • PAMAM dendrimer
  • folate receptor targeting
  • gene therapy
  • head and neck squamous cell carcinoma

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Folic acid-decorated polyamidoamine dendrimer mediates selective uptake and high expression of genes in head and neck cancer cells. / Xu, Leyuan; Kittrell, Shannon; Yeudall, W. Andrew; Yang, Hu.

In: Nanomedicine, Vol. 11, No. 22, 11.2016, p. 2959-2973.

Research output: Contribution to journalArticle

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