Forebrain NR2B overexpression facilitating the prefrontal cortex long-term potentiation and enhancing working memory function in mice

Yihui Cui, Jing Jin, Xuliang Zhang, Hao Xu, Liguo Yang, Dan Du, Qingwen Zeng, Joseph Zhuo Tsien, Huiting Yu, Xiaohua Cao

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Prefrontal cortex plays an important role in working memory, attention regulation and behavioral inhibition. Its functions are associated with NMDA receptors. However, there is little information regarding the roles of NMDA receptor NR2B subunit in prefrontal cortical synaptic plasticity and prefrontal cortex-related working memory. Whether the up-regulation of NR2B subunit influences prefrontal cortical synaptic plasticity and working memory is not yet clear. In the present study, we measured prefrontal cortical synaptic plasticity and working memory function in NR2B overexpressing transgenic mice. In vitro electrophysiological data showed that overexpression of NR2B specifically in the forebrain region resulted in enhancement of prefrontal cortical long-term potentiation (LTP) but did not alter long-term depression (LTD). The enhanced LTP was completely abolished by a NR2B subunit selective antagonist, Ro25-6981, indicating that overexpression of NR2B subunit is responsible for enhanced LTP. In addition, NR2B transgenic mice exhibited better performance in a set of working memory paradigms including delay no-match-to-place T-maze, working memory version of water maze and odor span task. Our study provides evidence that NR2B subunit of NMDA receptor in prefrontal cortex is critical for prefrontal cortex LTP and prefrontal cortex-related working memory.

Original languageEnglish (US)
Article numbere20312
JournalPloS one
Volume6
Issue number5
DOIs
StatePublished - Jun 6 2011

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Long-Term Potentiation
Prosencephalon
Prefrontal Cortex
Short-Term Memory
brain
Data storage equipment
mice
Neuronal Plasticity
Plasticity
N-Methyl-D-Aspartate Receptors
Transgenic Mice
receptors
genetically modified organisms
Odors
prefrontal cortex
antagonists
Up-Regulation
odors
Depression
Water

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Forebrain NR2B overexpression facilitating the prefrontal cortex long-term potentiation and enhancing working memory function in mice. / Cui, Yihui; Jin, Jing; Zhang, Xuliang; Xu, Hao; Yang, Liguo; Du, Dan; Zeng, Qingwen; Tsien, Joseph Zhuo; Yu, Huiting; Cao, Xiaohua.

In: PloS one, Vol. 6, No. 5, e20312, 06.06.2011.

Research output: Contribution to journalArticle

Cui, Yihui ; Jin, Jing ; Zhang, Xuliang ; Xu, Hao ; Yang, Liguo ; Du, Dan ; Zeng, Qingwen ; Tsien, Joseph Zhuo ; Yu, Huiting ; Cao, Xiaohua. / Forebrain NR2B overexpression facilitating the prefrontal cortex long-term potentiation and enhancing working memory function in mice. In: PloS one. 2011 ; Vol. 6, No. 5.
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