Forkhead containing transcription factor Albino controls tetrapyrrole-based body pigmentation in planarian

Chen Wang, Xiao Shuai Han, Fang Fang Li, Shuang Huang, Yong Wen Qin, Xian Xian Zhao, Qing Jing

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pigmentation processes occur from invertebrates to mammals. Owing to the complexity of the pigmentary system, in vivo animal models for pigmentation study are limited. Planarians are capable of regenerating any missing part including the dark-brown pigments, providing a promising model for pigmentation study. However, the molecular mechanism of planarian body pigmentation is poorly understood. We found in an RNA interference screen that a forkhead containing transcription factor, Albino, was required for pigmentation without affecting survival or other regeneration processes. In addition, the body color recovered after termination of Albino double stranded RNA feeding owing to the robust stem cell system. Further expression analysis revealed a spatial and temporal correlation between Albino and pigmentation process. Gene expression arrays revealed that the expression of three tetrapyrrole biosynthesis enzymes, ALAD, ALAS and PBGD, was impaired upon Albino RNA interference. RNA interference of PBGD led to a similar albinism phenotype caused by Albino RNA interference. Moreover, PBGD was specifically expressed in pigment cells and can serve as a pigment cell molecular marker. Our results revealed that Albino controls planarian body color pigmentation dominantly via regulating tetrapyrrole biogenesis. These results identified Albino as the key regulator of the tetrapyrrole-based planarian body pigmentation, suggesting a role of Albino during stem cell-pigment cell fate decision and provided new insights into porphyria pathogenesis.

Original languageEnglish (US)
Article number16029
JournalCell Discovery
Volume2
DOIs
StatePublished - Aug 2 2016

Fingerprint

Tetrapyrroles
Planarians
Forkhead Transcription Factors
Pigmentation
Pigments
Transcription Factors
RNA
RNA Interference
Stem cells
Color
Mammals
Double-Stranded RNA
Biosynthesis
Gene expression
Animals
Stem Cells
Albinism
Porphyrias
Enzymes
Invertebrates

Keywords

  • FoxP
  • PBGD
  • body color
  • pigmentation
  • planarian
  • tetrapyrrole

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Forkhead containing transcription factor Albino controls tetrapyrrole-based body pigmentation in planarian. / Wang, Chen; Han, Xiao Shuai; Li, Fang Fang; Huang, Shuang; Qin, Yong Wen; Zhao, Xian Xian; Jing, Qing.

In: Cell Discovery, Vol. 2, 16029, 02.08.2016.

Research output: Contribution to journalArticle

Wang, Chen ; Han, Xiao Shuai ; Li, Fang Fang ; Huang, Shuang ; Qin, Yong Wen ; Zhao, Xian Xian ; Jing, Qing. / Forkhead containing transcription factor Albino controls tetrapyrrole-based body pigmentation in planarian. In: Cell Discovery. 2016 ; Vol. 2.
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