Formation and structure of dentine in the rat incisor after chronic exposure to sodium fluoride

J. Appleton, David Henry Pashley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Weanling rats were chronically exposed to sodium fluoride by feeding them for eight weeks with a standard powdered diet incorporating sodium fluoride at 0.025%, 0.05% and 0.1% by weight. After eight weeks blood was removed by cardiac puncture and the levels of plasma calcium and phosphate determined. The incisor teeth were removed and their structure examined in the scanning electron microscope (SEM) using backscattered electron imaging to determine the relative concentration and distribution of the mineral phase in the dentine. There was no significant alteration to the normal serum calcium and phosphate levels. Small scattered interglobular spaces were seen in the incisor dentine of rats consuming the 0.025% sodium fluoride diet. The principle feature with the 0.05% diet were marked striations in the circumpulpal dentine but with the 0.1% diet there was severe disruption of dentine structure with continuous bands of interglobular spaces. These spaces were larger labially than lingually reflecting differences in the size and shape of calcospherites. The distribution of interglobular dentine would suggest that its formation takes time to establish.

Original languageEnglish (US)
Pages (from-to)711-719
Number of pages9
JournalScanning Microscopy
Volume8
Issue number3
StatePublished - Dec 1 1994

Fingerprint

sodium fluorides
diets
Nutrition
rats
Rats
Sodium
calcium
Calcium
phosphates
Phosphates
striation
teeth
serums
blood
Blood
Electron microscopes
Minerals
electron microscopes
minerals
Scanning

Keywords

  • Dentine
  • dentine formation
  • mineralisation
  • rat incisor
  • sodium fluoride

ASJC Scopus subject areas

  • Instrumentation

Cite this

Formation and structure of dentine in the rat incisor after chronic exposure to sodium fluoride. / Appleton, J.; Pashley, David Henry.

In: Scanning Microscopy, Vol. 8, No. 3, 01.12.1994, p. 711-719.

Research output: Contribution to journalArticle

Appleton, J. ; Pashley, David Henry. / Formation and structure of dentine in the rat incisor after chronic exposure to sodium fluoride. In: Scanning Microscopy. 1994 ; Vol. 8, No. 3. pp. 711-719.
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