Formation of proteasome-PA700 complexes directly correlates with activation of peptidase activity

George M. Adams, Brad Crotchett, Clive A. Slaughter, George N. DeMartino, Edward P. Gogol

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The proteolytic activity of the eukaryotic 20S proteasome is stimulated by a multisubunit activator, PA700, which forms both 1:1 and 2:1 complexes with the proteasome. Formation of the complexes is enhanced by an additional protein assembly called modulator, which also stimulates the enzymatic activity of the proteasome only in the presence of PA700. Here we show that the binding of PA700 to the proteasome is cooperative, as is the activation of the proteasome's intrinsic peptidase activity. Modulator increases the extent of complex formation and peptidase activation, while preserving the cooperative kinetics. Furthermore, the increase in activity is not linear with the number of PA700 assemblies bound to the proteasome, but rather with the number of proteasome-PA700 complexes, regardless of the PA700:proteasome stoichiometry. Hence the stimulation of peptidase activity is fully (or almost fully) effected by the binding of a single PA700 to the 20S proteasome. The stimulation of peptidase by modulator is explained entirely by the increased number of proteasome-PA700 complexes formed in its presence, rather than by any substantial direct stimulation of catalysis. These observations are consistent with a model in which PA700, either alone or assisted by modulator, promotes conformational changes in the proteasome that activate the catalytic sites and/or facilitate access of peptide substrates to these sites.

Original languageEnglish (US)
Pages (from-to)12927-12932
Number of pages6
JournalBiochemistry
Volume37
Issue number37
DOIs
StatePublished - Sep 15 1998
Externally publishedYes

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Proteasome Endopeptidase Complex
Peptide Hydrolases
Chemical activation
Modulators
PA700 proteasome activator
Catalysis
Stoichiometry
Catalytic Domain
Peptides
Kinetics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Formation of proteasome-PA700 complexes directly correlates with activation of peptidase activity. / Adams, George M.; Crotchett, Brad; Slaughter, Clive A.; DeMartino, George N.; Gogol, Edward P.

In: Biochemistry, Vol. 37, No. 37, 15.09.1998, p. 12927-12932.

Research output: Contribution to journalArticle

Adams, George M. ; Crotchett, Brad ; Slaughter, Clive A. ; DeMartino, George N. ; Gogol, Edward P. / Formation of proteasome-PA700 complexes directly correlates with activation of peptidase activity. In: Biochemistry. 1998 ; Vol. 37, No. 37. pp. 12927-12932.
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