Fractal binding and dissociation kinetics of heart-related compounds on biosensor surfaces

Atul Doke, Sunil Mathur, Ajit Sadana

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A fractal analysis is presented for the binding and dissociation of different heart-related compounds in solution to receptors immobilized on biosensor surfaces. The data analyzed include LCAT (lecithin cholesterol acyl transferase) concentrations in solution to egg white apoA-I rHDL immobilized on a biosensor chip surface (1), native, mildly oxidized, and strongly oxidized LDL in solution to a heparin-modified Au-surface of a surface plasmon resonance (SPR) biosensor (2), and TRITC-labeled HDL in solution to a bare optical fiber surface (3). Single-and dual-fractal models were used to fit the data. Values of the binding and the dissociation rate coefficient(s), affinity values, and the fractal dimensions were obtained from the regression analysis provided by Corel Quattro Pro 8.0 (4). The binding rate coefficients are quite sensitive to the degree of heterogeneity on the sensor chip surface. Predictive equations are developed for the binding rate coefficient as a function of the degree of heterogeneity present on the sensor chip surface and on the LCAT concentration in solution and for the affinity as a function of the ratio of fractal dimensions present in the binding and the dissociation phases. The analysis presented provided physical insights into these analyte-receptor reactions occurring on different biosensor surfaces.

Original languageEnglish (US)
Pages (from-to)337-357
Number of pages21
JournalJournal of Receptors and Signal Transduction
Volume26
Issue number4
DOIs
StatePublished - Aug 1 2006

Fingerprint

Fractals
Biosensing Techniques
Biosensors
Kinetics
Lecithins
Transferases
Fractal dimension
Cholesterol
Optical Fibers
Egg White
Surface Plasmon Resonance
Apolipoprotein A-I
Heparin
Sensors
Surface plasmon resonance
Regression Analysis
Regression analysis
Optical fibers

Keywords

  • Binding and dissociation kinetics
  • Biosensors
  • Fractals
  • Heart-related compounds

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fractal binding and dissociation kinetics of heart-related compounds on biosensor surfaces. / Doke, Atul; Mathur, Sunil; Sadana, Ajit.

In: Journal of Receptors and Signal Transduction, Vol. 26, No. 4, 01.08.2006, p. 337-357.

Research output: Contribution to journalArticle

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