Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations

Jamie Ann De Stefano, J. M. Foy, D. W. Sullivan, S. M. Dawes, M. T. Cushion, G. F. Babcock, R. G. Sleight, H. van Halbeek, P. D. Walzer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations of Pneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (>95%) enriched populations of P. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enriched P. carinii cell populations and should have important applications to research on this organism.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalParasitology Research
Volume80
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Pneumocystis carinii
fractionation
developmental stages
Population
Trophozoites
trophozoites
sorting
Cysts
lungs
viability
Lung
rats
organisms
Research
cells
methodology

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations. / De Stefano, Jamie Ann; Foy, J. M.; Sullivan, D. W.; Dawes, S. M.; Cushion, M. T.; Babcock, G. F.; Sleight, R. G.; van Halbeek, H.; Walzer, P. D.

In: Parasitology Research, Vol. 80, No. 1, 01.01.1994, p. 1-9.

Research output: Contribution to journalArticle

De Stefano, JA, Foy, JM, Sullivan, DW, Dawes, SM, Cushion, MT, Babcock, GF, Sleight, RG, van Halbeek, H & Walzer, PD 1994, 'Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations', Parasitology Research, vol. 80, no. 1, pp. 1-9. https://doi.org/10.1007/BF00932616
De Stefano, Jamie Ann ; Foy, J. M. ; Sullivan, D. W. ; Dawes, S. M. ; Cushion, M. T. ; Babcock, G. F. ; Sleight, R. G. ; van Halbeek, H. ; Walzer, P. D. / Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations. In: Parasitology Research. 1994 ; Vol. 80, No. 1. pp. 1-9.
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