Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations

J. A. De Stefano, J. M. Foy, D. W. Sullivan, S. M. Dawes, M. T. Cushion, G. F. Babcock, R. G. Sleight, H. van Halbeek, P. D. Walzer

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations of Pneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (>95%) enriched populations of P. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enriched P. carinii cell populations and should have important applications to research on this organism.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalParasitology Research
Volume80
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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    De Stefano, J. A., Foy, J. M., Sullivan, D. W., Dawes, S. M., Cushion, M. T., Babcock, G. F., Sleight, R. G., van Halbeek, H., & Walzer, P. D. (1994). Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations. Parasitology Research, 80(1), 1-9. https://doi.org/10.1007/BF00932616