Freeze-drying of mammalian sperm

Levent Keskintepe, Ali Eroglu

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Long-term preservation of mammalian sperm at suprazero temperatures is desired to save storage and space costs as well as to facilitate transport of preserved samples. This can be accomplished by the freezedrying of sperm samples. Although freeze-drying results in immotile and membrane-compromised sperm, intracytoplasmic sperm injection (ICSI) can be used to introduce such an immotile sperm into an oocyte and thus start the fertilization process. So far, it has been shown that improved freeze-drying protocols preserve chromosomal integrity and oocyte-activating factor(s) at 4 °C for several years and at ambient temperature for approximately 1 month, which permits shipping freeze-dried samples at ambient temperature. This chapter concisely reviews freeze-drying of mammalian sperm first and then presents a simple freeze-drying protocol.

Original languageEnglish (US)
Pages (from-to)489-497
Number of pages9
JournalMethods in Molecular Biology
Volume1257
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Keywords

  • Cryopreservation
  • Cryoprotectant
  • Freeze-drying
  • Freezing
  • ICSI
  • Intracytoplasmic sperm injection
  • Long-term storage
  • Sperm
  • Spermatozoa
  • Trehalose

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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