Frog Cytochrome P‐450 (11β,aldo), a Single Enzyme Involved in the Final Steps of Glucocorticoid and Mineralocorticoid Biosynthesis

Yasuki Nonaka, Hiroshi Takemori, Sunil Krishna Halder, Tiejun Sun, Miho Ohta, Osamu Hatano, Akira Takakusu, Mitsuhiro Okamoto

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A cDNA for cytochrome P ‐450(11β,aldo) was cloned from a library of bullfrog interrenal tissue (tissue corresponding to the mammalian adrenal gland). The 1919‐bp cDNA encoded a protein of 517 amino acids. Its amino acid sequence was highly similar to the sequences of bovine P ‐450(11β) and rat P ‐450(11β,aldo) when P ‐450(11β) family enzymes reported to date were examined. The enzyme expressed in COS7 cells had the 11β‐hydroxylation, 18‐hydroxylation activities and aldosterone synthetic activity. Northern‐blot and immunoblot analyses suggested that a single P ‐450(11β) enzyme was expressed in bullfrog interrenal tissue. These results suggest that a single enzyme catalyzes the final steps of glucocorticoid and mineralocorticoid biosynthesis in bullfrog interrenal tissue as in bovine adrenal gland. A phylogenetic tree of CYP11B genes suggests that the frog enzyme diverged at an earlier evolutionary time from other vertebrate enzymes. Immunohistochemical and in situ hybridization studies indicated that steroidogenic cells existed in the outer region of interrenal tissue more densely than in the inner region, whereas some medullary cells made clusters like islets. Most of the cells were diffusely distributed in the tissue.

Original languageEnglish (US)
Pages (from-to)249-256
Number of pages8
JournalEuropean Journal of Biochemistry
Volume229
Issue number1
DOIs
StatePublished - Jan 1 1995

Fingerprint

Mineralocorticoids
Biosynthesis
Anura
Cytochrome P-450 Enzyme System
Glucocorticoids
Tissue
Rana catesbeiana
Enzymes
Adrenal Glands
Complementary DNA
Amino Acids
Aldosterone
Libraries
In Situ Hybridization
Vertebrates
Rats
Amino Acid Sequence
Genes
Proteins

Keywords

  • CYP11B
  • Frog
  • aldosterone synthase
  • cytochrome P ‐450(11β)
  • expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Frog Cytochrome P‐450 (11β,aldo), a Single Enzyme Involved in the Final Steps of Glucocorticoid and Mineralocorticoid Biosynthesis. / Nonaka, Yasuki; Takemori, Hiroshi; Halder, Sunil Krishna; Sun, Tiejun; Ohta, Miho; Hatano, Osamu; Takakusu, Akira; Okamoto, Mitsuhiro.

In: European Journal of Biochemistry, Vol. 229, No. 1, 01.01.1995, p. 249-256.

Research output: Contribution to journalArticle

Nonaka, Yasuki ; Takemori, Hiroshi ; Halder, Sunil Krishna ; Sun, Tiejun ; Ohta, Miho ; Hatano, Osamu ; Takakusu, Akira ; Okamoto, Mitsuhiro. / Frog Cytochrome P‐450 (11β,aldo), a Single Enzyme Involved in the Final Steps of Glucocorticoid and Mineralocorticoid Biosynthesis. In: European Journal of Biochemistry. 1995 ; Vol. 229, No. 1. pp. 249-256.
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