Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

Yuen Keng Ng, Wing Sze Lau, Vivian Wai Yan Lui, Alfred Sze Lok Cheng, Patrick Kwok Shing Ng, Stephen Kwok Wing Tsui, Yue Sun Cheung, Paul Bo San Lai

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.

Original languageEnglish (US)
Pages (from-to)496-503
Number of pages8
JournalFEBS Letters
Volume587
Issue number5
DOIs
StatePublished - Mar 1 2013
Externally publishedYes

Keywords

  • Akt
  • Hep3B
  • Huh7
  • NORE1B
  • PLC/PRF/5
  • Staurosporine
  • Stk4

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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