Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

Yuen Keng Ng; Wing Sze Lau; Vivian Wai Yan Lui; Alfred Sze Lok Cheng; Patrick Kwok Shing Ng; Stephen Kwok Wing Tsui; Yue Sun Cheung; Paul Bo San Lai

doi:10.1016/j.febslet.2013.01.018

Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

Yuen Keng Ng, Wing Sze Lau, Vivian Wai Yan Lui, Alfred Sze Lok Cheng, Patrick Kwok Shing Ng, Stephen Kwok Wing Tsui, Yue Sun Cheung, Paul Bo San Lai

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.

Original language	English (US)
Pages (from-to)	496-503
Number of pages	8
Journal	FEBS Letters
Volume	587
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2013.01.018
State	Published - Mar 1 2013
Externally published	Yes

Keywords

Akt
Hep3B
Huh7
NORE1B
PLC/PRF/5
Staurosporine
Stk4

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells",

abstract = "The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.",

keywords = "Akt, Hep3B, Huh7, NORE1B, PLC/PRF/5, Staurosporine, Stk4",

author = "Ng, {Yuen Keng} and Lau, {Wing Sze} and Lui, {Vivian Wai Yan} and Cheng, {Alfred Sze Lok} and Ng, {Patrick Kwok Shing} and Tsui, {Stephen Kwok Wing} and Cheung, {Yue Sun} and Lai, {Paul Bo San}",

note = "Funding Information: This study is supported by the Direct Research Grant (2009.1.098) from the Chinese University of Hong Kong. The authors want to thank Dr. Yukiko Gotoh for providing us the myc-tagged full-length Mst1 mammalian expression plasmid. Thanks to Dr. Laura Stabile and Kathryn Supko for editing the manuscript. Also thanks to May Sui-Mui Li (Institute of Digestive Diseases) and the Surgical Research Laboratory of Department of Surgery, the Chinese University of Hong Kong for their technical supports. ",

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doi = "10.1016/j.febslet.2013.01.018",

language = "English (US)",

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AU - Ng, Yuen Keng

AU - Lau, Wing Sze

AU - Lui, Vivian Wai Yan

AU - Cheng, Alfred Sze Lok

AU - Ng, Patrick Kwok Shing

AU - Tsui, Stephen Kwok Wing

AU - Cheung, Yue Sun

AU - Lai, Paul Bo San

N1 - Funding Information: This study is supported by the Direct Research Grant (2009.1.098) from the Chinese University of Hong Kong. The authors want to thank Dr. Yukiko Gotoh for providing us the myc-tagged full-length Mst1 mammalian expression plasmid. Thanks to Dr. Laura Stabile and Kathryn Supko for editing the manuscript. Also thanks to May Sui-Mui Li (Institute of Digestive Diseases) and the Surgical Research Laboratory of Department of Surgery, the Chinese University of Hong Kong for their technical supports.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.

AB - The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.

KW - Akt

KW - Hep3B

KW - Huh7

KW - NORE1B

KW - PLC/PRF/5

KW - Staurosporine

KW - Stk4

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ER -

Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

Abstract

Keywords

ASJC Scopus subject areas

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