TY - JOUR
T1 - Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity
AU - Corbella, Paola
AU - Moskophidis, Demetrius
AU - Spanopoulou, Eugenia
AU - Mamalaki, Clio
AU - Tolaini, Mauro
AU - Itano, Andrea
AU - Lans, Deborah
AU - Baltimore, David
AU - Robeyj, Ellen
AU - Kioussis, Dimitris
PY - 1994/7
Y1 - 1994/7
N2 - Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.
AB - Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.
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U2 - 10.1016/1074-7613(94)90078-7
DO - 10.1016/1074-7613(94)90078-7
M3 - Article
C2 - 7889414
AN - SCOPUS:0028467791
VL - 1
SP - 269
EP - 276
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 4
ER -