Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity

Paola Corbella, Dimitrios Moskofidis, Eugenia Spanopoulou, Clio Mamalaki, Mauro Tolaini, Andrea Itano, Deborah Lans, David Baltimore, Ellen Robeyj, Dimitris Kioussis

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.

Original languageEnglish (US)
Pages (from-to)269-276
Number of pages8
JournalImmunity
Volume1
Issue number4
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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T-Cell Antigen Receptor Specificity
Cell Lineage
Helper-Inducer T-Lymphocytes
Thymocytes
T-Cell Antigen Receptor
Transgenes
Genetic Recombination
T-Lymphocytes
Phenotype
Antigens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity. / Corbella, Paola; Moskofidis, Dimitrios; Spanopoulou, Eugenia; Mamalaki, Clio; Tolaini, Mauro; Itano, Andrea; Lans, Deborah; Baltimore, David; Robeyj, Ellen; Kioussis, Dimitris.

In: Immunity, Vol. 1, No. 4, 01.01.1994, p. 269-276.

Research output: Contribution to journalArticle

Corbella, P, Moskofidis, D, Spanopoulou, E, Mamalaki, C, Tolaini, M, Itano, A, Lans, D, Baltimore, D, Robeyj, E & Kioussis, D 1994, 'Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity', Immunity, vol. 1, no. 4, pp. 269-276. https://doi.org/10.1016/1074-7613(94)90078-7
Corbella, Paola ; Moskofidis, Dimitrios ; Spanopoulou, Eugenia ; Mamalaki, Clio ; Tolaini, Mauro ; Itano, Andrea ; Lans, Deborah ; Baltimore, David ; Robeyj, Ellen ; Kioussis, Dimitris. / Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity. In: Immunity. 1994 ; Vol. 1, No. 4. pp. 269-276.
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