Abstract
Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 269-276 |
| Number of pages | 8 |
| Journal | Immunity |
| Volume | 1 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases
Cite this
Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity. / Corbella, Paola; Moskophidis, Demetrius; Spanopoulou, Eugenia; Mamalaki, Clio; Tolaini, Mauro; Itano, Andrea; Lans, Deborah; Baltimore, David; Robeyj, Ellen; Kioussis, Dimitris.
In: Immunity, Vol. 1, No. 4, 07.1994, p. 269-276.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity
AU - Corbella, Paola
AU - Moskophidis, Demetrius
AU - Spanopoulou, Eugenia
AU - Mamalaki, Clio
AU - Tolaini, Mauro
AU - Itano, Andrea
AU - Lans, Deborah
AU - Baltimore, David
AU - Robeyj, Ellen
AU - Kioussis, Dimitris
PY - 1994/7
Y1 - 1994/7
N2 - Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.
AB - Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.
UR - http://www.scopus.com/inward/record.url?scp=0028467791&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028467791&partnerID=8YFLogxK
U2 - 10.1016/1074-7613(94)90078-7
DO - 10.1016/1074-7613(94)90078-7
M3 - Article
C2 - 7889414
AN - SCOPUS:0028467791
VL - 1
SP - 269
EP - 276
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 4
ER -