Functional human corneal equivalents constructed from cell lines

May Griffith, Rosemarie Osborne, Rejean Hunger, Xiaojuan Xiong, Charles J. Doillon, Noelani L.C. Laycock, Malik Hakim, Ying Song, Mitchell A. Watsky

Research output: Contribution to journalArticle

386 Citations (Scopus)

Abstract

Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked hum an corneas in key physical and physiological functions including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.

Original languageEnglish (US)
Pages (from-to)2169-2172
Number of pages4
JournalScience
Volume286
Issue number5447
DOIs
StatePublished - Dec 10 1999

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Cornea
Cell Line
Ion Transport
Drug-Related Side Effects and Adverse Reactions
Endothelium
Epithelium
Biomarkers
Gene Expression
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Griffith, M., Osborne, R., Hunger, R., Xiong, X., Doillon, C. J., Laycock, N. L. C., ... Watsky, M. A. (1999). Functional human corneal equivalents constructed from cell lines. Science, 286(5447), 2169-2172. https://doi.org/10.1126/science.286.5447.2169

Functional human corneal equivalents constructed from cell lines. / Griffith, May; Osborne, Rosemarie; Hunger, Rejean; Xiong, Xiaojuan; Doillon, Charles J.; Laycock, Noelani L.C.; Hakim, Malik; Song, Ying; Watsky, Mitchell A.

In: Science, Vol. 286, No. 5447, 10.12.1999, p. 2169-2172.

Research output: Contribution to journalArticle

Griffith, M, Osborne, R, Hunger, R, Xiong, X, Doillon, CJ, Laycock, NLC, Hakim, M, Song, Y & Watsky, MA 1999, 'Functional human corneal equivalents constructed from cell lines', Science, vol. 286, no. 5447, pp. 2169-2172. https://doi.org/10.1126/science.286.5447.2169
Griffith M, Osborne R, Hunger R, Xiong X, Doillon CJ, Laycock NLC et al. Functional human corneal equivalents constructed from cell lines. Science. 1999 Dec 10;286(5447):2169-2172. https://doi.org/10.1126/science.286.5447.2169
Griffith, May ; Osborne, Rosemarie ; Hunger, Rejean ; Xiong, Xiaojuan ; Doillon, Charles J. ; Laycock, Noelani L.C. ; Hakim, Malik ; Song, Ying ; Watsky, Mitchell A. / Functional human corneal equivalents constructed from cell lines. In: Science. 1999 ; Vol. 286, No. 5447. pp. 2169-2172.
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