Functional Identification of a Novel Transport System for Endogenous and Synthetic Opioid Peptides in the Rabbit Conjunctival Epithelial Cell Line CJVE

Sudha Ananth, Senthil Karunakaran, Pamela M. Martin, Chandrasekharam N. Nagineni, John J. Hooks, Sylvia B. Smith, Puttur D. Prasad, Vadivel Ganapathy

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Purpose: To investigate whether conjunctival epithelial cells express transport processes for opioid peptides. Methods: We monitored the uptake of [3H]deltorphin II and [3H]DADLE, two hydrolysis-resistant synthetic opioid peptides, in the rabbit conjunctival epithelial cell line CJVE and elucidated the characteristics of the uptake process. Results: CJVE cells express robust uptake activity for deltorphin II and DADLE. Both opioid peptides compete with each other for transport. Several endogenous and synthetic opioid peptides, but not non-peptide opioid antagonists, are recognized by the transport process. Though various peptides inhibit the uptake of deltorphin II and DADLE in a similar manner, the uptake of deltorphin II is partly Na +-dependent whereas that of DADLE mostly Na+-independent. The transport process shows high affinity for many endogenous/synthetic opioid peptides. Functional features reveal that this transport process may be distinct from the opioid peptide transport system described in the retinal pigment epithelial cell line ARPE-19 and also from the organic anion transporting polypeptides, which are known to transport opioid peptides. Conclusions: CJVE cells express a novel, hitherto unknown transport process for endogenous/synthetic opioid peptides. This new transport process may offer an effective delivery route for opioid peptide drugs to the posterior segment of the eye.

Original languageEnglish (US)
Pages (from-to)1226-1235
Number of pages10
JournalPharmaceutical Research
Volume26
Issue number5
DOIs
StatePublished - May 2009

Keywords

  • Conjunctival epithelial cell
  • Non-peptide opioid antagonists
  • Opioid peptides
  • Organic anion transporting polypeptides
  • Transport process

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

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