Functional NOS 1 in the rat mesenteric arterial bed

Jennifer C Sullivan, Ararat D. Giulumian, David M. Pollock, Leslie C. Fuchs, Jennifer S. Pollock

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previously we have demonstrated functional nitric oxide synthase (NOS) 1 in large arteries. Because resistance arteries largely determine blood pressure, this study examined whether functional NOS 1 also exists in resistance arteries. Phenylephrine (PE) contraction was measured in the absence and presence of the NOS 1 inhibitor N5-(1-imino-3-butenyl)-L-ornithine (VNIO) in isolated mesenteric resistance arteries (endothelium intact and denuded) from Sprague-Dawley rats. For NOS 1 activity and expression, the mesenteric arterial bed was separated into cytosolic and particulate fractions. NOS activity was assayed by measuring the conversion of [3H]arginine to [3H]citrulline inhibited by a nonselective NOS inhibitor or VNIO. VNIO increased PE sensitivity in endothelium-intact and -denuded arteries. In cytosolic and particulate fractions of the arterial bed, ∼40% of NOS activity was inhibited by VNIO. Immunoprecipitation and Western blot analysis revealed two NOS 1 immunoreactive bands. One band corresponded to the rat brain isoform, whereas the second was of a slightly lower molecular mass. The cytosolic fraction contained both isoforms; however, the particulate fraction had only the lower molecular mass form. These studies demonstrate the existence of functional NOS 1 in resistance arteries.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume283
Issue number2 52-2
StatePublished - Jul 30 2002

Fingerprint

Nitric Oxide Synthase
Arteries
Phenylephrine
Endothelium
Protein Isoforms
Citrulline
Mesenteric Arteries
Ornithine
Immunoprecipitation
Sprague Dawley Rats
Arginine
Western Blotting
Blood Pressure
Brain

Keywords

  • Neuronal nitric oxide synthase activity
  • Resistance arteries
  • Subcellular fractionation
  • Vascular reactivity

ASJC Scopus subject areas

  • Physiology

Cite this

Sullivan, J. C., Giulumian, A. D., Pollock, D. M., Fuchs, L. C., & Pollock, J. S. (2002). Functional NOS 1 in the rat mesenteric arterial bed. American Journal of Physiology - Heart and Circulatory Physiology, 283(2 52-2).

Functional NOS 1 in the rat mesenteric arterial bed. / Sullivan, Jennifer C; Giulumian, Ararat D.; Pollock, David M.; Fuchs, Leslie C.; Pollock, Jennifer S.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 283, No. 2 52-2, 30.07.2002.

Research output: Contribution to journalArticle

Sullivan, JC, Giulumian, AD, Pollock, DM, Fuchs, LC & Pollock, JS 2002, 'Functional NOS 1 in the rat mesenteric arterial bed', American Journal of Physiology - Heart and Circulatory Physiology, vol. 283, no. 2 52-2.
Sullivan, Jennifer C ; Giulumian, Ararat D. ; Pollock, David M. ; Fuchs, Leslie C. ; Pollock, Jennifer S. / Functional NOS 1 in the rat mesenteric arterial bed. In: American Journal of Physiology - Heart and Circulatory Physiology. 2002 ; Vol. 283, No. 2 52-2.
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