Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line

Carlos M Isales, Bauer Sumpio, Roni Jacob Bollag, Qing Zhong, Kehong Ding, Wei Du, Jose Rodriguez-Commes, Raquel Lopez, Oscar R. Rosales, Jose Gasalla-Herraiz, Richard McCarthy, Paula Q. Barrett

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Acute parathyroid hormone exposure induces vascular smooth muscle relaxation. In contrast, continuous infusion of parathyroid hormone leads to vasoconstriction and an elevation of blood pressure. Despite the known effects of parathyroid hormone on vascular smooth muscle, possible direct effects on the vascular endothelium have not previously been investigated. Using a human umbilical vein endothelial cell line, we found that parathyroid hormone increased both intracellular calcium and cellular cAMP content in these endothelial cells. Furthermore, exposure of these cells to increasing concentrations of parathyroid hormone stimulated both [3H]thymidine incorporation and endothelin-1 secretion. Parathyroid hormone/parathyroid hormone-related peptide receptor mRNA could be detected at low levels in these cells. In summary, these data demonstrate that endothelium-derived cells contain functional parathyroid hormone receptors. The potential physiological role of these receptors remains to be determined.

Original languageEnglish (US)
Pages (from-to)E654-E662
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume279
Issue number3 42-3
DOIs
StatePublished - Jan 1 2000

Keywords

  • Adenosine 3',5'-cyclic monophosphate
  • Endothelial cells
  • Intracellular calcium
  • Parathyroid hormone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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