Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line

Carlos M Isales, Bauer Sumpio, Roni Jacob Bollag, Qing Zhong, Kehong Ding, Wei Du, Jose Rodriguez-Commes, Raquel Lopez, Oscar R. Rosales, Jose Gasalla-Herraiz, Richard McCarthy, Paula Q. Barrett

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Acute parathyroid hormone exposure induces vascular smooth muscle relaxation. In contrast, continuous infusion of parathyroid hormone leads to vasoconstriction and an elevation of blood pressure. Despite the known effects of parathyroid hormone on vascular smooth muscle, possible direct effects on the vascular endothelium have not previously been investigated. Using a human umbilical vein endothelial cell line, we found that parathyroid hormone increased both intracellular calcium and cellular cAMP content in these endothelial cells. Furthermore, exposure of these cells to increasing concentrations of parathyroid hormone stimulated both [3H]thymidine incorporation and endothelin-1 secretion. Parathyroid hormone/parathyroid hormone-related peptide receptor mRNA could be detected at low levels in these cells. In summary, these data demonstrate that endothelium-derived cells contain functional parathyroid hormone receptors. The potential physiological role of these receptors remains to be determined.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume279
Issue number3 42-3
StatePublished - Oct 4 2000

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Parathyroid Hormone Receptors
Umbilical Veins
Parathyroid Hormone
Endothelial Cells
Cell Line
Vascular Smooth Muscle
Parathyroid Hormone Receptor Type 1
Muscle Relaxation
Human Umbilical Vein Endothelial Cells
Vascular Endothelium
Endothelin-1
Vasoconstriction
Thymidine
Endothelium
Blood Pressure
Calcium
Messenger RNA

Keywords

  • Adenosine 3',5'-cyclic monophosphate
  • Endothelial cells
  • Intracellular calcium
  • Parathyroid hormone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line. / Isales, Carlos M; Sumpio, Bauer; Bollag, Roni Jacob; Zhong, Qing; Ding, Kehong; Du, Wei; Rodriguez-Commes, Jose; Lopez, Raquel; Rosales, Oscar R.; Gasalla-Herraiz, Jose; McCarthy, Richard; Barrett, Paula Q.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 279, No. 3 42-3, 04.10.2000.

Research output: Contribution to journalArticle

Isales, CM, Sumpio, B, Bollag, RJ, Zhong, Q, Ding, K, Du, W, Rodriguez-Commes, J, Lopez, R, Rosales, OR, Gasalla-Herraiz, J, McCarthy, R & Barrett, PQ 2000, 'Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line', American Journal of Physiology - Endocrinology and Metabolism, vol. 279, no. 3 42-3.
Isales, Carlos M ; Sumpio, Bauer ; Bollag, Roni Jacob ; Zhong, Qing ; Ding, Kehong ; Du, Wei ; Rodriguez-Commes, Jose ; Lopez, Raquel ; Rosales, Oscar R. ; Gasalla-Herraiz, Jose ; McCarthy, Richard ; Barrett, Paula Q. / Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line. In: American Journal of Physiology - Endocrinology and Metabolism. 2000 ; Vol. 279, No. 3 42-3.
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