Functional significance of cytosolic endothelial nitric-oxide synthase (eNOS)

Regulation of hyperpermeability

Fabiola A. Sánchez, Roshniben Rana, Francisco G. González, Toru Iwahashi, Ricardo G. Durán, David J Fulton, Annie V. Beuve, David D. Kim, Walter N. Durán

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Endothelial NOS (eNOS)-derived NO is a key factor in regulating microvascular permeability. We demonstrated previously that eNOS translocation from the plasma membrane to the cytosol is required for hyperpermeability. Herein, we tested the hypothesis that eNOS activation in the cytosol is necessary for agonist-induced hyperpermeability. To study the fundamental properties of endothelial cell monolayer permeability, we generated ECV-304 cells that stably express cDNA constructs targeting eNOS to the cytosol or plasma membrane. eNOS-transfected ECV-304 cells recapitulate the eNOS translocation and permeability properties of postcapillary venular endothelial cells (Sánchez, F. A., Rana, R., Kim, D. D., Iwahashi, T., Zheng, R., Lal, B. K., Gordon, D. M., Meininger, C. J., and Durán, W. N. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 6849-6853). We used platelet-activating factor (PAF) as a proinflammatory agonist. PAF activated eNOS by increasing phosphorylation of Ser-1177 and inducing dephosphorylation of Thr-495, increasing NO production, and elevating permeability to FITC-dextran 70 in monolayers of cells expressing wild-type and cytosolic eNOS. PAF failed to increase permeability to FITC-dextran 70 in monolayers of cells transfected with eNOS targeted to the plasma membrane. Interestingly, this occurred despite eNOS Ser-1177 phosphorylation and production of comparable amounts of NO. Our results demonstrate that the presence of eNOS in the cytosol is necessary for PAF-induced hyperpermeability. Our data provide new insights into the dynamics of eNOS and eNOS-derived NO in the process of inflammation.

Original languageEnglish (US)
Pages (from-to)30409-30414
Number of pages6
JournalJournal of Biological Chemistry
Volume286
Issue number35
DOIs
StatePublished - Sep 2 2011

Fingerprint

Nitric Oxide Synthase Type III
Platelet Activating Factor
Cytosol
Permeability
Cell membranes
Monolayers
Phosphorylation
Endothelial cells
Cell Membrane
Dextrans
Endothelial Cells
Cells
Capillary Permeability
Complementary DNA
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Functional significance of cytosolic endothelial nitric-oxide synthase (eNOS) : Regulation of hyperpermeability. / Sánchez, Fabiola A.; Rana, Roshniben; González, Francisco G.; Iwahashi, Toru; Durán, Ricardo G.; Fulton, David J; Beuve, Annie V.; Kim, David D.; Durán, Walter N.

In: Journal of Biological Chemistry, Vol. 286, No. 35, 02.09.2011, p. 30409-30414.

Research output: Contribution to journalArticle

Sánchez, FA, Rana, R, González, FG, Iwahashi, T, Durán, RG, Fulton, DJ, Beuve, AV, Kim, DD & Durán, WN 2011, 'Functional significance of cytosolic endothelial nitric-oxide synthase (eNOS): Regulation of hyperpermeability', Journal of Biological Chemistry, vol. 286, no. 35, pp. 30409-30414. https://doi.org/10.1074/jbc.M111.234294
Sánchez, Fabiola A. ; Rana, Roshniben ; González, Francisco G. ; Iwahashi, Toru ; Durán, Ricardo G. ; Fulton, David J ; Beuve, Annie V. ; Kim, David D. ; Durán, Walter N. / Functional significance of cytosolic endothelial nitric-oxide synthase (eNOS) : Regulation of hyperpermeability. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 35. pp. 30409-30414.
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