Fungal endophthalmitis diagnosis by detection of Candida albicans DNA in intraocular fluid by use of a species-specific polymerase chain reaction assay

Jose A. Hidalgo, George J. Alangaden, Dean Eliott, Robert A. Akins, James Puklin, Gary Abrams, Jose Antonio Vazquez

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Candida endophthalmitis is a serious infection secondary to hematogenous dissemination or direct inoculation of the organisms following trauma or eye surgery. The diagnosis is based on the characteristic findings in the infected eye and on culture of vitreous samples. Unfortunately, the yield of vitreous cultures is limited. The use of a Candida albicans species-specific polymerase chain reaction (PCR) assay in the diagnosis of Candida endophthalmitis is reported herein. Four patients with suspected fungal endophthalmitis underwent vitrectomy for diagnostic and therapeutic purposes. In 2 of the 4, vitreous cultures were negative. However, characteristic PCR products were generated in all 4 patient specimens, enabling the rapid diagnosis of Candida endophthalmitis in all 4. Clinical response was observed in all cases. These results demonstrate the utility of PCR-mediated detection of C. albicans in vitreous samples.

Original languageEnglish (US)
Pages (from-to)1198-1201
Number of pages4
JournalJournal of Infectious Diseases
Volume181
Issue number3
DOIs
StatePublished - Apr 19 2000

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Endophthalmitis
Aqueous Humor
Candida albicans
Candida
Polymerase Chain Reaction
DNA
Vitrectomy
Coinfection
Wounds and Injuries
Therapeutics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Fungal endophthalmitis diagnosis by detection of Candida albicans DNA in intraocular fluid by use of a species-specific polymerase chain reaction assay. / Hidalgo, Jose A.; Alangaden, George J.; Eliott, Dean; Akins, Robert A.; Puklin, James; Abrams, Gary; Vazquez, Jose Antonio.

In: Journal of Infectious Diseases, Vol. 181, No. 3, 19.04.2000, p. 1198-1201.

Research output: Contribution to journalArticle

Hidalgo, Jose A. ; Alangaden, George J. ; Eliott, Dean ; Akins, Robert A. ; Puklin, James ; Abrams, Gary ; Vazquez, Jose Antonio. / Fungal endophthalmitis diagnosis by detection of Candida albicans DNA in intraocular fluid by use of a species-specific polymerase chain reaction assay. In: Journal of Infectious Diseases. 2000 ; Vol. 181, No. 3. pp. 1198-1201.
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