Fungicidal lipid-transfer peptide from Daucus carota sativa seeds

A. Yili, H. A. Aisa, X. Imamu, R. H. Zhen, Q. Zhang, V. V. Maksimov, O. N. Veshkurova, Sh I. Salikhov

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A non-specific lipid-transfer peptide (nsLTP) with fungicidal activity was isolated from Daucus carota sativa carrot seeds. Peptides were purified by a method including aqueous extraction, anion-exchange chromatography over CM-TSK-650M, and HPLC over a column of 250/8/4 Protein@Peptide C 18 using an acetonitrile gradient. The molecular weight of the peptide was determined as 9624 Da by mass spectrometry. The peptide was found to have fungicidal activity against the pathogenic fungus Verticillium dahliae. The partial N-terminal sequence, which was highly homologous to the N-terminal sequences of lipid-transfer peptides from seeds of rice, tobacco, and maize, was determined using Edman automated sequencing.

Original languageEnglish (US)
Pages (from-to)450-453
Number of pages4
JournalChemistry of Natural Compounds
Volume43
Issue number4
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

Keywords

  • Carrot seeds
  • Fungicidal lipid-transfer peptides
  • N-terminal amino-acid sequence

ASJC Scopus subject areas

  • Plant Science

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