Further characterization of embryonic stem cell-derived radial glial cells

Sean S. Liour, Stacey A. Kraemer, Michael B. Dinkins, Chen Ying Su, Makoto Yanagisawa, Robert K. Yu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Previously, we showed that radial glia-like (RG) cells differentiated from embryonic stem (ES) cells after retinoic acid induction (Liour and Yu, 2003: Glia 42:109-117). In the present study, we demonstrate that the production of RG cells from ES cells is independent of the neural differentiation protocol used. These ES cell-derived RG (ES-RG) cells are similar in morphology to RG cells in vivo and express several characteristic RG cell markers. The processes of these ES-RG cells are organized into radial arrays similar to the RG scaffold in developing CNS. Expression of Pax6, along with other circumstantial data, suggests that at least some of these ES-RG cells are neural progenitors. The progression of neurogenesis into gliogenesis during the in vitro neural differentiation of ES cells recapitulates the in vivo developmental process. The identification of two cell surface markers, SSEA-1 and GM1, on both the native embryonic RG cells and ES-RG cells, may facilitate purification of radial glial cells for future studies and cell therapy. Overall, our study suggests that differentiation of radial glial cells is a common pathway during the neural differentiation of ES cells.

Original languageEnglish (US)
Pages (from-to)43-56
Number of pages14
JournalGlia
Volume53
Issue number1
DOIs
StatePublished - Jan 1 2006

Fingerprint

Ependymoglial Cells
Embryonic Stem Cells
Neuroglia
CD15 Antigens
Neural Pathways
Neurogenesis
Cell- and Tissue-Based Therapy
Tretinoin
Stem Cells

Keywords

  • Embryonic stem cell
  • Gliogenesis
  • Neural progenitor
  • Neurogenesis
  • Radial glia

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Liour, S. S., Kraemer, S. A., Dinkins, M. B., Su, C. Y., Yanagisawa, M., & Yu, R. K. (2006). Further characterization of embryonic stem cell-derived radial glial cells. Glia, 53(1), 43-56. https://doi.org/10.1002/glia.20257

Further characterization of embryonic stem cell-derived radial glial cells. / Liour, Sean S.; Kraemer, Stacey A.; Dinkins, Michael B.; Su, Chen Ying; Yanagisawa, Makoto; Yu, Robert K.

In: Glia, Vol. 53, No. 1, 01.01.2006, p. 43-56.

Research output: Contribution to journalArticle

Liour, SS, Kraemer, SA, Dinkins, MB, Su, CY, Yanagisawa, M & Yu, RK 2006, 'Further characterization of embryonic stem cell-derived radial glial cells', Glia, vol. 53, no. 1, pp. 43-56. https://doi.org/10.1002/glia.20257
Liour SS, Kraemer SA, Dinkins MB, Su CY, Yanagisawa M, Yu RK. Further characterization of embryonic stem cell-derived radial glial cells. Glia. 2006 Jan 1;53(1):43-56. https://doi.org/10.1002/glia.20257
Liour, Sean S. ; Kraemer, Stacey A. ; Dinkins, Michael B. ; Su, Chen Ying ; Yanagisawa, Makoto ; Yu, Robert K. / Further characterization of embryonic stem cell-derived radial glial cells. In: Glia. 2006 ; Vol. 53, No. 1. pp. 43-56.
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