Further characterization of tetracycline's quantitative binding to dentin

Alfred E. Ciarlone, Richard D. Johnson, David Henry Pashley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Unerupted human third molar crown segments were used to measure the change in the concentration of 3H-tetracycline that occurs when it moves across dentin from pulpal to occlusal surfaces. When 1.25, 2.5, and 5×10-5M3H-tetracycline were filtered across dentin, binding increased by 21, 51, and 69%, respectively. When the same concentrations of 3H-tetracycline were preloaded onto crown segments and eluted with 1.1×10-3M nonradioactive tetracycline, the percentage of elution of 3H-tetracycline was not significantly different over time. When preloaded 3H-tetracycline was displaced by diffusion with water or 1.1×10-3M nonradioactive tetracycline, there were no significant differences. There was a significant difference between the rate of displacement of 1.25×10-5M3H-tetracycline and the other two preloaded concentrations when 5.8×10-4M3H-tetracycline/tetracycline was used as the eluant. These data suggest that the binding of 3H-tetracycline to dentin was concentration dependent and that 3H-tetracycline binds nonspecifically and reversibly.

Original languageEnglish (US)
Pages (from-to)335-338
Number of pages4
JournalJournal of Endodontics
Volume15
Issue number8
DOIs
StatePublished - Jan 1 1989

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Dentin
Tetracycline
Crowns
Third Molar

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Further characterization of tetracycline's quantitative binding to dentin. / Ciarlone, Alfred E.; Johnson, Richard D.; Pashley, David Henry.

In: Journal of Endodontics, Vol. 15, No. 8, 01.01.1989, p. 335-338.

Research output: Contribution to journalArticle

Ciarlone, Alfred E. ; Johnson, Richard D. ; Pashley, David Henry. / Further characterization of tetracycline's quantitative binding to dentin. In: Journal of Endodontics. 1989 ; Vol. 15, No. 8. pp. 335-338.
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