TY - JOUR
T1 - G-protein and tyrosine kinase receptor cross-talk in rat aortic smooth muscle cells
T2 - Thrombin- and angiotensin II-induced tyrosine phosphorylation of insulin receptor substrate-1 and insulin-like growth factor 1 receptor
AU - Du, Jie
AU - Sperling, Laurence S.
AU - Marrero, Mario B.
AU - Phillips, Liz
AU - Delafontaine, Patrick
N1 - Funding Information:
We thank Kate W. Harris for editorial support. This research was supported by funds from the National Institutes of Health (NIH), the American Heart Association (AHA) and the Georgia Chapter of the AHA.
PY - 1996/1/26
Y1 - 1996/1/26
N2 - Insulin-like growth factor I is an autocrine/paracrine factor for vascular smooth muscle cells and is required for angiotensin II- and thrombin-induced mitogenesis. The insulin-like growth factor I-triggered signaling pathway involves autophosphorylation of the β-subunit of its tyrosine kinase receptor and phosphorylation of insulin receptor substrate-1, the latter providing binding sites for proteins with src homology-2 domains. In rat aortic smooth muscle cells we observed that both angiotensin II and thrombin induced rapid tyrosine phosphorylation of insulin receptor substrate-1. Our results also demonstrated that these mitogens rapidly stimulated phosphorylation of the insulin-like growth factor I receptor β-chain. These data demonstrate a novel interaction between the G-protein coupled angiotensin II and thrombin receptors and the tyrosine-kinase insulin-like growth factor I receptor.
AB - Insulin-like growth factor I is an autocrine/paracrine factor for vascular smooth muscle cells and is required for angiotensin II- and thrombin-induced mitogenesis. The insulin-like growth factor I-triggered signaling pathway involves autophosphorylation of the β-subunit of its tyrosine kinase receptor and phosphorylation of insulin receptor substrate-1, the latter providing binding sites for proteins with src homology-2 domains. In rat aortic smooth muscle cells we observed that both angiotensin II and thrombin induced rapid tyrosine phosphorylation of insulin receptor substrate-1. Our results also demonstrated that these mitogens rapidly stimulated phosphorylation of the insulin-like growth factor I receptor β-chain. These data demonstrate a novel interaction between the G-protein coupled angiotensin II and thrombin receptors and the tyrosine-kinase insulin-like growth factor I receptor.
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U2 - 10.1006/bbrc.1996.0165
DO - 10.1006/bbrc.1996.0165
M3 - Article
C2 - 8579617
AN - SCOPUS:0030058802
SN - 0006-291X
VL - 218
SP - 934
EP - 939
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -