G-protein and tyrosine kinase receptor cross-talk in rat aortic smooth muscle cells: Thrombin- and angiotensin II-induced tyrosine phosphorylation of insulin receptor substrate-1 and insulin-like growth factor 1 receptor

Jie Du, Laurence S. Sperling, Mario B Marrero, Liz Phillips, Patrick Delafontaine

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Insulin-like growth factor I is an autocrine/paracrine factor for vascular smooth muscle cells and is required for angiotensin II- and thrombin-induced mitogenesis. The insulin-like growth factor I-triggered signaling pathway involves autophosphorylation of the β-subunit of its tyrosine kinase receptor and phosphorylation of insulin receptor substrate-1, the latter providing binding sites for proteins with src homology-2 domains. In rat aortic smooth muscle cells we observed that both angiotensin II and thrombin induced rapid tyrosine phosphorylation of insulin receptor substrate-1. Our results also demonstrated that these mitogens rapidly stimulated phosphorylation of the insulin-like growth factor I receptor β-chain. These data demonstrate a novel interaction between the G-protein coupled angiotensin II and thrombin receptors and the tyrosine-kinase insulin-like growth factor I receptor.

Original languageEnglish (US)
Pages (from-to)934-939
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number3
DOIs
StatePublished - Jan 26 1996

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Receptor Cross-Talk
Somatomedin Receptors
Insulin Receptor Substrate Proteins
Phosphorylation
Receptor Protein-Tyrosine Kinases
Somatomedins
GTP-Binding Proteins
Thrombin
Angiotensin II
Smooth Muscle Myocytes
Tyrosine
Muscle
Rats
IGF Type 1 Receptor
Cells
Insulin-Like Growth Factor I
Thrombin Receptors
Angiotensin Receptors
src Homology Domains
Vascular Smooth Muscle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

G-protein and tyrosine kinase receptor cross-talk in rat aortic smooth muscle cells : Thrombin- and angiotensin II-induced tyrosine phosphorylation of insulin receptor substrate-1 and insulin-like growth factor 1 receptor. / Du, Jie; Sperling, Laurence S.; Marrero, Mario B; Phillips, Liz; Delafontaine, Patrick.

In: Biochemical and Biophysical Research Communications, Vol. 218, No. 3, 26.01.1996, p. 934-939.

Research output: Contribution to journalArticle

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