Abstract
In addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as "traffic cops" to coordinate receptor targeting to specific locations or as "signal transducers" to directly mediate receptor signal propagation. As revealed in protein-protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α2B- and β2-adrenegic receptors with the small GTPases Rab8 and ARF1.
Original language | English (US) |
---|---|
Title of host publication | G Protein Coupled ReceptorsModeling, Activation, Interactions and Virtual Screening |
Publisher | Academic Press Inc. |
Pages | 97-108 |
Number of pages | 12 |
ISBN (Print) | 9780124078659 |
DOIs | |
State | Published - Jan 1 2013 |
Publication series
Name | Methods in Enzymology |
---|---|
Volume | 522 |
ISSN (Print) | 0076-6879 |
ISSN (Electronic) | 1557-7988 |
Fingerprint
Keywords
- ARF1
- G-protein-coupled receptor
- Protein-protein interaction
- Rab8
- Signaling
- Small GTPases
- Trafficking
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
G-protein-coupled receptor interaction with small GTPases. / Dong, Chunmin; Wu, Guangyu.
G Protein Coupled ReceptorsModeling, Activation, Interactions and Virtual Screening. Academic Press Inc., 2013. p. 97-108 (Methods in Enzymology; Vol. 522).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - G-protein-coupled receptor interaction with small GTPases
AU - Dong, Chunmin
AU - Wu, Guangyu
PY - 2013/1/1
Y1 - 2013/1/1
N2 - In addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as "traffic cops" to coordinate receptor targeting to specific locations or as "signal transducers" to directly mediate receptor signal propagation. As revealed in protein-protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α2B- and β2-adrenegic receptors with the small GTPases Rab8 and ARF1.
AB - In addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as "traffic cops" to coordinate receptor targeting to specific locations or as "signal transducers" to directly mediate receptor signal propagation. As revealed in protein-protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α2B- and β2-adrenegic receptors with the small GTPases Rab8 and ARF1.
KW - ARF1
KW - G-protein-coupled receptor
KW - Protein-protein interaction
KW - Rab8
KW - Signaling
KW - Small GTPases
KW - Trafficking
UR - http://www.scopus.com/inward/record.url?scp=84873366173&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873366173&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-407865-9.00006-6
DO - 10.1016/B978-0-12-407865-9.00006-6
M3 - Chapter
C2 - 23374182
AN - SCOPUS:84873366173
SN - 9780124078659
T3 - Methods in Enzymology
SP - 97
EP - 108
BT - G Protein Coupled ReceptorsModeling, Activation, Interactions and Virtual Screening
PB - Academic Press Inc.
ER -