TY - JOUR
T1 - G Protein Function during Biomembrane Fusion in Dictyostelium
T2 - Presence and Importance of a Gαs Subunit during Fertilization and Phagocytosis
AU - Browning, Darren D
AU - O’day, Danton H.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Previous work has shown that GTPase function is essential for fertilization and cannibalistic phagocytosis during the sexual development of Dictyostelium discoideum . In this work, the importance of heterotrimeric G proteins during these events was established further using aluminum fluoride which inhibited both fertilization and cannibalistic phagocytosis, as well as the phagocytosis of bacteria by vegetative amoebae. Using distinct immune sera directed against the amino terminus and the carboxy terminus of mammalian Gαs, we have provided unique evidence for a Gαs subunit of approximately 55 kDa in D. discoideum (referred to as dGαs). Furthermore, this protein localizes to the membranes of fusing cells as well as to both vegetative and zygote giant cells, indicating that it might function during fertilization as well as during both vegetative (i.e., bacterial) and cannibalistic (i.e., amoebal) phagocytosis. During its down-regulation in nonphagocytic cells new isozymes of dGαs appear, suggesting that it may be posttranslationally modified. Having identified a putitive Gαs homologue, this work has set the stage for further investigations into its function in Dictyostelium.
AB - Previous work has shown that GTPase function is essential for fertilization and cannibalistic phagocytosis during the sexual development of Dictyostelium discoideum . In this work, the importance of heterotrimeric G proteins during these events was established further using aluminum fluoride which inhibited both fertilization and cannibalistic phagocytosis, as well as the phagocytosis of bacteria by vegetative amoebae. Using distinct immune sera directed against the amino terminus and the carboxy terminus of mammalian Gαs, we have provided unique evidence for a Gαs subunit of approximately 55 kDa in D. discoideum (referred to as dGαs). Furthermore, this protein localizes to the membranes of fusing cells as well as to both vegetative and zygote giant cells, indicating that it might function during fertilization as well as during both vegetative (i.e., bacterial) and cannibalistic (i.e., amoebal) phagocytosis. During its down-regulation in nonphagocytic cells new isozymes of dGαs appear, suggesting that it may be posttranslationally modified. Having identified a putitive Gαs homologue, this work has set the stage for further investigations into its function in Dictyostelium.
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U2 - 10.1006/excr.1995.1282
DO - 10.1006/excr.1995.1282
M3 - Article
C2 - 7641821
AN - SCOPUS:0029116785
SN - 0014-4827
VL - 219
SP - 709
EP - 716
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -