Ganglioside GD2 specificity of monoclonal antibodies to human neuroblastoma cell

Megumi Saito, Robert K Yu, N. K.V. Cheung

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Four monoclonal antibodies (3F8, 3A7, 3G6, and 2F7) against human neuroblastoma cells have been suggested to react with surface glycolipids of these cells. In this report these monoclonal antibodies were shown to be specific to the disialoganglioside GD2 using a thin-layer chromatography (TLC)-immunostaining method. When mixed human brain gangliosides were developed by TLC in two different solvent systems and incubated with each of the monoclonal antibodies, only GD2 was stained. These antibodies also reacted with highly purified GD2 on the plate. These findings suggest that GD2 provides an antigenic site on the surface of human neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume127
Issue number1
DOIs
StatePublished - Feb 28 1985
Externally publishedYes

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Neuroblastoma
Thin layer chromatography
Monoclonal Antibodies
Thin Layer Chromatography
Gangliosides
Glycolipids
Brain
Antibodies
GD2 ganglioside

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ganglioside GD2 specificity of monoclonal antibodies to human neuroblastoma cell. / Saito, Megumi; Yu, Robert K; Cheung, N. K.V.

In: Biochemical and Biophysical Research Communications, Vol. 127, No. 1, 28.02.1985, p. 1-7.

Research output: Contribution to journalArticle

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