Gangliosides GM1 and GD(1b) are antigens for IgM M-protein in a patient with motor neuron disease

L. Freddo, Robert K Yu, N. Latov

Research output: Contribution to journalArticle

217 Scopus citations

Abstract

We studied a patient with an IgM M-protein and lower motor neuron disease to identify the antigens to which the M-protein bound. Gangliosides from peripheral nerve and spinal cord were separated by high-performance thin-layer chromatography and immunostained with the patient's serum. The serum IgM immunostained two gangliosides identified as GM1 and GD(1b), and immunostaining was specific for the M-protein light chain type. IgM-binding to the two gangliosides was detectable by ELISA at serum dilutions of greater than 1:10,000, and the M-protein was selectively immunoabsorbed by liposomes containing GM1 or GD(1b). The IgM M-protein also bound to asialo-GM1, indicating reactivity to the galactosyl(β1-3)N-acetylgalactosaminyl moiety shared by GM1, GD(1b), and asialo-GM1.

Original languageEnglish (US)
Pages (from-to)454-458
Number of pages5
JournalNeurology
Volume36
Issue number4
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

ASJC Scopus subject areas

  • Clinical Neurology

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