Gangliosides of human, bovine, and rabbit plasma

Robert K Yu, R. W. Ledeen

Research output: Contribution to journalArticle

271 Citations (Scopus)

Abstract

Gangliosides were isolated from human, bovine, and rabbit plasma and were quantified by gas liquid chromatography. Purification was achieved by sequential use of partitioning in solvents, DEAE Sephadex chromatography, base treatment, and silicic acid chromatography. Human and bovine plasma yielded slightly more than 1 μmole of lipid bound sialic acid/100 ml; for rabbit plasma the value was 0.28 μmole/100 ml. The total bovine plasma ganglioside fraction contained equal amounts of N acetylneuraminic and N glycolylneuraminic acids; rabbit plasma gangliosides had about 1% of the latter, and the human plasma sample contained only the former. Thin layer chromatography revealed important differences among the plasmas from the three species, but all possessed hematosides and hexosamine containing gangliosides. The approximate ratios of these two categories, based on sialic acid content, were (hematosides: hexosamine type): human, 2:1; rabbit, 3:2; and bovine, 2:3. The fatty acid compositions of both categories were characteristic of extraneural gangliosides and included six major acids: palmitic, stearic, behenic, tricosanoic, lignoceric, and nervonic. The major long chain base in each sample was sphingosine, while only a trace of the C20 isomer was detected.

Original languageEnglish (US)
Pages (from-to)680-686
Number of pages7
JournalJournal of Lipid Research
Volume13
Issue number5
StatePublished - Dec 1 1972

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Gangliosides
Rabbits
Plasmas
G(M3) Ganglioside
Hexosamines
N-Acetylneuraminic Acid
Chromatography
Palmitic Acids
Silicic Acid
Plasma (human)
DEAE-Dextran
Thin layer chromatography
Stearic Acids
Sphingosine
Liquid chromatography
Isomers
Gas chromatography
Purification
Thin Layer Chromatography
Gas Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Gangliosides of human, bovine, and rabbit plasma. / Yu, Robert K; Ledeen, R. W.

In: Journal of Lipid Research, Vol. 13, No. 5, 01.12.1972, p. 680-686.

Research output: Contribution to journalArticle

Yu, RK & Ledeen, RW 1972, 'Gangliosides of human, bovine, and rabbit plasma', Journal of Lipid Research, vol. 13, no. 5, pp. 680-686.
Yu, Robert K ; Ledeen, R. W. / Gangliosides of human, bovine, and rabbit plasma. In: Journal of Lipid Research. 1972 ; Vol. 13, No. 5. pp. 680-686.
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